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Development of a reproducible porcine model of infected burn wounds

Severe burns are traumatic and physically debilitating injuries with a high rate of mortality. Bacterial infections often complicate burn injuries, which presents unique challenges for wound management and improved patient outcomes. Currently, pigs are used as the gold standard of pre-clinical model...

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Autores principales: Al-deen Said, Sayf, Jatana, Samreen, Ponti, András K., Johnson, Erin E., Such, Kimberly A., Zangara, Megan T., Madajka, Maria, Papay, Francis, McDonald, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058257/
https://www.ncbi.nlm.nih.gov/pubmed/35510036
http://dx.doi.org/10.14440/jbm.2022.379
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author Al-deen Said, Sayf
Jatana, Samreen
Ponti, András K.
Johnson, Erin E.
Such, Kimberly A.
Zangara, Megan T.
Madajka, Maria
Papay, Francis
McDonald, Christine
author_facet Al-deen Said, Sayf
Jatana, Samreen
Ponti, András K.
Johnson, Erin E.
Such, Kimberly A.
Zangara, Megan T.
Madajka, Maria
Papay, Francis
McDonald, Christine
author_sort Al-deen Said, Sayf
collection PubMed
description Severe burns are traumatic and physically debilitating injuries with a high rate of mortality. Bacterial infections often complicate burn injuries, which presents unique challenges for wound management and improved patient outcomes. Currently, pigs are used as the gold standard of pre-clinical models to study infected skin wounds due to the similarity between porcine and human skin in terms of structure and immunological response. However, utilizing this large animal model for wound infection studies can be technically challenging and create issues with data reproducibility. We present a detailed protocol for a porcine model of infected burn wounds based on our experience in creating and evaluating full thickness burn wounds infected with Staphylococcus aureus on six pigs. Wound healing kinetics and bacterial clearance were measured over a period of 27 d in this model. Enumerated are steps to achieve standardized wound creation, bacterial inoculation, and dressing techniques. Systematic evaluation of wound healing and bacterial colonization of the wound bed is also described. Finally, advice on animal housing considerations, efficient bacterial plating procedures, and overcoming common technical challenges is provided. This protocol aims to provide investigators with a step-by-step guide to execute a technically challenging porcine wound infection model in a reproducible manner. Accordingly, this would allow for the design and evaluation of more effective burn infection therapies leading to better strategies for patient care.
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spelling pubmed-90582572022-05-03 Development of a reproducible porcine model of infected burn wounds Al-deen Said, Sayf Jatana, Samreen Ponti, András K. Johnson, Erin E. Such, Kimberly A. Zangara, Megan T. Madajka, Maria Papay, Francis McDonald, Christine J Biol Methods Protocol Severe burns are traumatic and physically debilitating injuries with a high rate of mortality. Bacterial infections often complicate burn injuries, which presents unique challenges for wound management and improved patient outcomes. Currently, pigs are used as the gold standard of pre-clinical models to study infected skin wounds due to the similarity between porcine and human skin in terms of structure and immunological response. However, utilizing this large animal model for wound infection studies can be technically challenging and create issues with data reproducibility. We present a detailed protocol for a porcine model of infected burn wounds based on our experience in creating and evaluating full thickness burn wounds infected with Staphylococcus aureus on six pigs. Wound healing kinetics and bacterial clearance were measured over a period of 27 d in this model. Enumerated are steps to achieve standardized wound creation, bacterial inoculation, and dressing techniques. Systematic evaluation of wound healing and bacterial colonization of the wound bed is also described. Finally, advice on animal housing considerations, efficient bacterial plating procedures, and overcoming common technical challenges is provided. This protocol aims to provide investigators with a step-by-step guide to execute a technically challenging porcine wound infection model in a reproducible manner. Accordingly, this would allow for the design and evaluation of more effective burn infection therapies leading to better strategies for patient care. Journal of Biological Methods 2022-02-21 /pmc/articles/PMC9058257/ /pubmed/35510036 http://dx.doi.org/10.14440/jbm.2022.379 Text en © 2013-2022 The Journal of Biological Methods, All rights reserved. https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License: http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle Protocol
Al-deen Said, Sayf
Jatana, Samreen
Ponti, András K.
Johnson, Erin E.
Such, Kimberly A.
Zangara, Megan T.
Madajka, Maria
Papay, Francis
McDonald, Christine
Development of a reproducible porcine model of infected burn wounds
title Development of a reproducible porcine model of infected burn wounds
title_full Development of a reproducible porcine model of infected burn wounds
title_fullStr Development of a reproducible porcine model of infected burn wounds
title_full_unstemmed Development of a reproducible porcine model of infected burn wounds
title_short Development of a reproducible porcine model of infected burn wounds
title_sort development of a reproducible porcine model of infected burn wounds
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058257/
https://www.ncbi.nlm.nih.gov/pubmed/35510036
http://dx.doi.org/10.14440/jbm.2022.379
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