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Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids

Hydrogels with concave microwells are one of the simplest means to obtain uniform-sized cellular spheroids. However, the inherent swelling of hydrogels leads to reduced mechanical strength and thus deforms the structure of the microwells. In this study, we developed a hydrogel with microwells for fo...

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Detalles Bibliográficos
Autores principales: Li, Yingjun, Wang, Ying, Shen, Chong, Meng, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058638/
https://www.ncbi.nlm.nih.gov/pubmed/35517174
http://dx.doi.org/10.1039/d0ra06188c
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author Li, Yingjun
Wang, Ying
Shen, Chong
Meng, Qin
author_facet Li, Yingjun
Wang, Ying
Shen, Chong
Meng, Qin
author_sort Li, Yingjun
collection PubMed
description Hydrogels with concave microwells are one of the simplest means to obtain uniform-sized cellular spheroids. However, the inherent swelling of hydrogels leads to reduced mechanical strength and thus deforms the structure of the microwells. In this study, we developed a hydrogel with microwells for formation of vascular spheroids via non-swellable di-acrylated Pluronic F127 (F127-DA), which showed higher mechanical strength than a conventional di-acrylated polyethylene glycol (PEG-DA) hydrogel. The uniform-sized vascular spheroids were spontaneously generated by human umbilical vein endothelial cells (HUVECs) and fibroblasts in the microwells. The endothelial functions of vascular spheroids were about 1-fold higher than those in two-dimensional (2D) culture, as indicated by secretion of nitric oxide (NO), prostacyclin (PGI2) and tissue factor pathway inhibitor (TFPI). Interestingly, the vascular spheroids with large diameter showed higher sensitivity to ethanol toxicity than those with small diameter, possibly due to the higher endothelial functions of large spheroids. Hence, F127-DA hydrogel with concave microwells provides a convenient way of forming uniform-sized spheroids that are useful for high throughput screening of drug/food toxicity.
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spelling pubmed-90586382022-05-04 Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids Li, Yingjun Wang, Ying Shen, Chong Meng, Qin RSC Adv Chemistry Hydrogels with concave microwells are one of the simplest means to obtain uniform-sized cellular spheroids. However, the inherent swelling of hydrogels leads to reduced mechanical strength and thus deforms the structure of the microwells. In this study, we developed a hydrogel with microwells for formation of vascular spheroids via non-swellable di-acrylated Pluronic F127 (F127-DA), which showed higher mechanical strength than a conventional di-acrylated polyethylene glycol (PEG-DA) hydrogel. The uniform-sized vascular spheroids were spontaneously generated by human umbilical vein endothelial cells (HUVECs) and fibroblasts in the microwells. The endothelial functions of vascular spheroids were about 1-fold higher than those in two-dimensional (2D) culture, as indicated by secretion of nitric oxide (NO), prostacyclin (PGI2) and tissue factor pathway inhibitor (TFPI). Interestingly, the vascular spheroids with large diameter showed higher sensitivity to ethanol toxicity than those with small diameter, possibly due to the higher endothelial functions of large spheroids. Hence, F127-DA hydrogel with concave microwells provides a convenient way of forming uniform-sized spheroids that are useful for high throughput screening of drug/food toxicity. The Royal Society of Chemistry 2020-12-16 /pmc/articles/PMC9058638/ /pubmed/35517174 http://dx.doi.org/10.1039/d0ra06188c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Yingjun
Wang, Ying
Shen, Chong
Meng, Qin
Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title_full Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title_fullStr Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title_full_unstemmed Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title_short Non-swellable F127-DA hydrogel with concave microwells for formation of uniform-sized vascular spheroids
title_sort non-swellable f127-da hydrogel with concave microwells for formation of uniform-sized vascular spheroids
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058638/
https://www.ncbi.nlm.nih.gov/pubmed/35517174
http://dx.doi.org/10.1039/d0ra06188c
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