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Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice culture

Utilization of live animals for mechanistic study is challenging yet pivotal to elucidate pathogenesis of neurological diseases. Here, we present a protocol that employs cultured brain slices derived from adult mice to examine mRNA metabolism. We describe the preparation of acute brain slices and th...

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Detalles Bibliográficos
Autores principales: Dzhala, Volodymyr, Fowler, Alan J., DiMarzio, Britt A., Staley, Kevin J., Suh, Jaehong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059153/
https://www.ncbi.nlm.nih.gov/pubmed/35509975
http://dx.doi.org/10.1016/j.xpro.2022.101349