Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead

3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be appl...

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Detalles Bibliográficos
Autores principales: Chrenek, Josie, Kirsch, Rebecca, Scheck, Kali, Willerth, Stephanie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059157/
https://www.ncbi.nlm.nih.gov/pubmed/35509974
http://dx.doi.org/10.1016/j.xpro.2022.101348
Descripción
Sumario:3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling in vitro.

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