Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead

3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be appl...

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Detalles Bibliográficos
Autores principales: Chrenek, Josie, Kirsch, Rebecca, Scheck, Kali, Willerth, Stephanie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059157/
https://www.ncbi.nlm.nih.gov/pubmed/35509974
http://dx.doi.org/10.1016/j.xpro.2022.101348
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author Chrenek, Josie
Kirsch, Rebecca
Scheck, Kali
Willerth, Stephanie M.
author_facet Chrenek, Josie
Kirsch, Rebecca
Scheck, Kali
Willerth, Stephanie M.
author_sort Chrenek, Josie
collection PubMed
description 3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling in vitro.
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spelling pubmed-90591572022-05-03 Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead Chrenek, Josie Kirsch, Rebecca Scheck, Kali Willerth, Stephanie M. STAR Protoc Protocol 3D bioprinting—a type of additive manufacturing—can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences’ fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling in vitro. Elsevier 2022-04-22 /pmc/articles/PMC9059157/ /pubmed/35509974 http://dx.doi.org/10.1016/j.xpro.2022.101348 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Chrenek, Josie
Kirsch, Rebecca
Scheck, Kali
Willerth, Stephanie M.
Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title_full Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title_fullStr Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title_full_unstemmed Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title_short Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead
title_sort protocol for printing 3d neural tissues using the bio x equipped with a pneumatic printhead
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059157/
https://www.ncbi.nlm.nih.gov/pubmed/35509974
http://dx.doi.org/10.1016/j.xpro.2022.101348
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