Parallel G-quadruplex-mediated protein dimerization and activation

We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed...

Descripción completa

Detalles Bibliográficos
Autores principales: Thi Truong, Tuom Tinh, Cao, Cuong, Dang, Dung Thanh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059161/
https://www.ncbi.nlm.nih.gov/pubmed/35518224
http://dx.doi.org/10.1039/d0ra06173e
_version_ 1784698254586281984
author Thi Truong, Tuom Tinh
Cao, Cuong
Dang, Dung Thanh
author_facet Thi Truong, Tuom Tinh
Cao, Cuong
Dang, Dung Thanh
author_sort Thi Truong, Tuom Tinh
collection PubMed
description We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed in the presence of parallel G4, which indicated that parallel G4 can induce dimerization and activation of proteins. This novel approach holds a great promise for studying G4-targeting functional dimeric proteins in celllular biology.
format Online
Article
Text
id pubmed-9059161
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90591612022-05-04 Parallel G-quadruplex-mediated protein dimerization and activation Thi Truong, Tuom Tinh Cao, Cuong Dang, Dung Thanh RSC Adv Chemistry We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed in the presence of parallel G4, which indicated that parallel G4 can induce dimerization and activation of proteins. This novel approach holds a great promise for studying G4-targeting functional dimeric proteins in celllular biology. The Royal Society of Chemistry 2020-08-13 /pmc/articles/PMC9059161/ /pubmed/35518224 http://dx.doi.org/10.1039/d0ra06173e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Thi Truong, Tuom Tinh
Cao, Cuong
Dang, Dung Thanh
Parallel G-quadruplex-mediated protein dimerization and activation
title Parallel G-quadruplex-mediated protein dimerization and activation
title_full Parallel G-quadruplex-mediated protein dimerization and activation
title_fullStr Parallel G-quadruplex-mediated protein dimerization and activation
title_full_unstemmed Parallel G-quadruplex-mediated protein dimerization and activation
title_short Parallel G-quadruplex-mediated protein dimerization and activation
title_sort parallel g-quadruplex-mediated protein dimerization and activation
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059161/
https://www.ncbi.nlm.nih.gov/pubmed/35518224
http://dx.doi.org/10.1039/d0ra06173e
work_keys_str_mv AT thitruongtuomtinh parallelgquadruplexmediatedproteindimerizationandactivation
AT caocuong parallelgquadruplexmediatedproteindimerizationandactivation
AT dangdungthanh parallelgquadruplexmediatedproteindimerizationandactivation

Ejemplares similares