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Purification and characterization of alkaline phosphatase from lactic acid bacteria

Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides. This study reported the first purification and characterization of ALP from LAB. The molecular weight of ALP was estimated to be 43 kDa measured by SDS-PAGE. The activity of...

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Autores principales: Chu, Yu-Hao, Yu, Xin-Xin, Jin, Xing, Wang, Yu-Tang, Zhao, Duo-Jia, Zhang, Po, Sun, Guang-Mei, Zhang, Ying-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059361/
https://www.ncbi.nlm.nih.gov/pubmed/35521616
http://dx.doi.org/10.1039/c8ra08921c
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author Chu, Yu-Hao
Yu, Xin-Xin
Jin, Xing
Wang, Yu-Tang
Zhao, Duo-Jia
Zhang, Po
Sun, Guang-Mei
Zhang, Ying-Hua
author_facet Chu, Yu-Hao
Yu, Xin-Xin
Jin, Xing
Wang, Yu-Tang
Zhao, Duo-Jia
Zhang, Po
Sun, Guang-Mei
Zhang, Ying-Hua
author_sort Chu, Yu-Hao
collection PubMed
description Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides. This study reported the first purification and characterization of ALP from LAB. The molecular weight of ALP was estimated to be 43 kDa measured by SDS-PAGE. The activity of purified enzyme was determined with the binding of p-nitrophenyl phosphate as the substrate. The results showed that the optimal temperature for ALP activity was 37 °C, and the optimal pH was 8.5. But ALP was stable at temperatures below 32 °C. The ALP activity remained at 80% when the pH was 8–9.5. The enzyme activity could be activated by Mg(2+), Ca(2+), and inhibited by Cu(2+), Zn(2+), and EDTA. The Michaelis–Menten constant was 6.05 mg kg(−1) with dimethoate as the substrate according to the Lineweaver–Burk plots. These results highlight an important potential use of ALP from LAB for the cleanup of pesticide pollution in raw materials for the food industry.
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spelling pubmed-90593612022-05-04 Purification and characterization of alkaline phosphatase from lactic acid bacteria Chu, Yu-Hao Yu, Xin-Xin Jin, Xing Wang, Yu-Tang Zhao, Duo-Jia Zhang, Po Sun, Guang-Mei Zhang, Ying-Hua RSC Adv Chemistry Alkaline phosphatase (ALP) excreted from lactic acid bacteria (LAB) showed the ability to degrade organophosphorus pesticides. This study reported the first purification and characterization of ALP from LAB. The molecular weight of ALP was estimated to be 43 kDa measured by SDS-PAGE. The activity of purified enzyme was determined with the binding of p-nitrophenyl phosphate as the substrate. The results showed that the optimal temperature for ALP activity was 37 °C, and the optimal pH was 8.5. But ALP was stable at temperatures below 32 °C. The ALP activity remained at 80% when the pH was 8–9.5. The enzyme activity could be activated by Mg(2+), Ca(2+), and inhibited by Cu(2+), Zn(2+), and EDTA. The Michaelis–Menten constant was 6.05 mg kg(−1) with dimethoate as the substrate according to the Lineweaver–Burk plots. These results highlight an important potential use of ALP from LAB for the cleanup of pesticide pollution in raw materials for the food industry. The Royal Society of Chemistry 2019-01-02 /pmc/articles/PMC9059361/ /pubmed/35521616 http://dx.doi.org/10.1039/c8ra08921c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Chu, Yu-Hao
Yu, Xin-Xin
Jin, Xing
Wang, Yu-Tang
Zhao, Duo-Jia
Zhang, Po
Sun, Guang-Mei
Zhang, Ying-Hua
Purification and characterization of alkaline phosphatase from lactic acid bacteria
title Purification and characterization of alkaline phosphatase from lactic acid bacteria
title_full Purification and characterization of alkaline phosphatase from lactic acid bacteria
title_fullStr Purification and characterization of alkaline phosphatase from lactic acid bacteria
title_full_unstemmed Purification and characterization of alkaline phosphatase from lactic acid bacteria
title_short Purification and characterization of alkaline phosphatase from lactic acid bacteria
title_sort purification and characterization of alkaline phosphatase from lactic acid bacteria
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059361/
https://www.ncbi.nlm.nih.gov/pubmed/35521616
http://dx.doi.org/10.1039/c8ra08921c
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