Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M(−1) s(−1), which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with h...

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Detalles Bibliográficos
Autores principales: Cai, Xuekang, Wang, Dan, Gao, Yasi, Yi, Long, Yang, Xing, Xi, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059488/
https://www.ncbi.nlm.nih.gov/pubmed/35521584
http://dx.doi.org/10.1039/c8ra09303b
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author Cai, Xuekang
Wang, Dan
Gao, Yasi
Yi, Long
Yang, Xing
Xi, Zhen
author_facet Cai, Xuekang
Wang, Dan
Gao, Yasi
Yi, Long
Yang, Xing
Xi, Zhen
author_sort Cai, Xuekang
collection PubMed
description We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M(−1) s(−1), which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.
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spelling pubmed-90594882022-05-04 Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells Cai, Xuekang Wang, Dan Gao, Yasi Yi, Long Yang, Xing Xi, Zhen RSC Adv Chemistry We developed a fast strain-promoted azide–alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M(−1) s(−1), which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials. The Royal Society of Chemistry 2018-12-19 /pmc/articles/PMC9059488/ /pubmed/35521584 http://dx.doi.org/10.1039/c8ra09303b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Cai, Xuekang
Wang, Dan
Gao, Yasi
Yi, Long
Yang, Xing
Xi, Zhen
Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title_full Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title_fullStr Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title_full_unstemmed Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title_short Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
title_sort tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059488/
https://www.ncbi.nlm.nih.gov/pubmed/35521584
http://dx.doi.org/10.1039/c8ra09303b
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