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DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer
Gastric cancer is still among the leading causes of cancer deaths worldwide. Despite the improvements in diagnostic methods, the status of early detection has not been achieved so far. Early diagnosis of gastric cancer may significantly improve the cure rate of patients. Therefore, a new diagnostic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059574/ https://www.ncbi.nlm.nih.gov/pubmed/35517584 http://dx.doi.org/10.1039/c8ra08642g |
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author | Zheng, Yue Zhao, Yunwang Di, Ya Xiu, Chenlin He, Lei Liao, Shiqi Li, Dongdong Huang, Baihai |
author_facet | Zheng, Yue Zhao, Yunwang Di, Ya Xiu, Chenlin He, Lei Liao, Shiqi Li, Dongdong Huang, Baihai |
author_sort | Zheng, Yue |
collection | PubMed |
description | Gastric cancer is still among the leading causes of cancer deaths worldwide. Despite the improvements in diagnostic methods, the status of early detection has not been achieved so far. Early diagnosis of gastric cancer may significantly improve the cure rate of patients. Therefore, a new diagnostic method is needed. In this study, subtractive SELEX was performed to screen gastric cancer serum-specific DNA aptamers by using gastric cancer serum and normal serum as the target and negative serum, respectively. Four highly specific aptamers generated for gastric cancer serum, Seq-3, Seq-6, Seq-19 and Seq-54, were developed using whole-serum subtractive SELEX technology with K(d) of 128 ± 26.3 nM, 149 ± 23.6 nM, 232 ± 44.2 nM, 202 ± 25.6 nM, respectively. These generated aptamers showed higher specificities toward their target serum by differentiating normal serum but closely related other cancer serums. The selected four high affinity DNA aptamers were further applied to the development based on qPCR method for the early detection of gastric cancer. In addition, we performed MALDI-TOF MS followed by secondary peptide sequencing MS analysis for the identification of the aptamer binding proteins. Among these potential biomarkers, APOA1, APOA4, PARD3, Importin subunit alpha-1 showed a relatively high score probability. Therefore, these four ssDNA aptamers generated in our study could be a promising molecular probe for gastric cancer diagnosis. |
format | Online Article Text |
id | pubmed-9059574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90595742022-05-04 DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer Zheng, Yue Zhao, Yunwang Di, Ya Xiu, Chenlin He, Lei Liao, Shiqi Li, Dongdong Huang, Baihai RSC Adv Chemistry Gastric cancer is still among the leading causes of cancer deaths worldwide. Despite the improvements in diagnostic methods, the status of early detection has not been achieved so far. Early diagnosis of gastric cancer may significantly improve the cure rate of patients. Therefore, a new diagnostic method is needed. In this study, subtractive SELEX was performed to screen gastric cancer serum-specific DNA aptamers by using gastric cancer serum and normal serum as the target and negative serum, respectively. Four highly specific aptamers generated for gastric cancer serum, Seq-3, Seq-6, Seq-19 and Seq-54, were developed using whole-serum subtractive SELEX technology with K(d) of 128 ± 26.3 nM, 149 ± 23.6 nM, 232 ± 44.2 nM, 202 ± 25.6 nM, respectively. These generated aptamers showed higher specificities toward their target serum by differentiating normal serum but closely related other cancer serums. The selected four high affinity DNA aptamers were further applied to the development based on qPCR method for the early detection of gastric cancer. In addition, we performed MALDI-TOF MS followed by secondary peptide sequencing MS analysis for the identification of the aptamer binding proteins. Among these potential biomarkers, APOA1, APOA4, PARD3, Importin subunit alpha-1 showed a relatively high score probability. Therefore, these four ssDNA aptamers generated in our study could be a promising molecular probe for gastric cancer diagnosis. The Royal Society of Chemistry 2019-01-09 /pmc/articles/PMC9059574/ /pubmed/35517584 http://dx.doi.org/10.1039/c8ra08642g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Zheng, Yue Zhao, Yunwang Di, Ya Xiu, Chenlin He, Lei Liao, Shiqi Li, Dongdong Huang, Baihai DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title | DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title_full | DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title_fullStr | DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title_full_unstemmed | DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title_short | DNA aptamers from whole-serum SELEX as new diagnostic agents against gastric cancer |
title_sort | dna aptamers from whole-serum selex as new diagnostic agents against gastric cancer |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059574/ https://www.ncbi.nlm.nih.gov/pubmed/35517584 http://dx.doi.org/10.1039/c8ra08642g |
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