One step DNA amplification of mammalian cells in picoliter microwell arrays

We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

Detalles Bibliográficos
Autores principales: Liu, Wenwen, Li, Zhao, Liu, Yuanjie, Wei, Qingquan, Liu, Yong, Ren, Lufeng, Wang, Chenyu, Yu, Yude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059946/
https://www.ncbi.nlm.nih.gov/pubmed/35520517
http://dx.doi.org/10.1039/c8ra06717a
Descripción
Sumario:We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

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