One step DNA amplification of mammalian cells in picoliter microwell arrays

We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.

Detalles Bibliográficos
Autores principales: Liu, Wenwen, Li, Zhao, Liu, Yuanjie, Wei, Qingquan, Liu, Yong, Ren, Lufeng, Wang, Chenyu, Yu, Yude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059946/
https://www.ncbi.nlm.nih.gov/pubmed/35520517
http://dx.doi.org/10.1039/c8ra06717a
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author Liu, Wenwen
Li, Zhao
Liu, Yuanjie
Wei, Qingquan
Liu, Yong
Ren, Lufeng
Wang, Chenyu
Yu, Yude
author_facet Liu, Wenwen
Li, Zhao
Liu, Yuanjie
Wei, Qingquan
Liu, Yong
Ren, Lufeng
Wang, Chenyu
Yu, Yude
author_sort Liu, Wenwen
collection PubMed
description We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.
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spelling pubmed-90599462022-05-04 One step DNA amplification of mammalian cells in picoliter microwell arrays Liu, Wenwen Li, Zhao Liu, Yuanjie Wei, Qingquan Liu, Yong Ren, Lufeng Wang, Chenyu Yu, Yude RSC Adv Chemistry We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy. The Royal Society of Chemistry 2019-01-21 /pmc/articles/PMC9059946/ /pubmed/35520517 http://dx.doi.org/10.1039/c8ra06717a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Liu, Wenwen
Li, Zhao
Liu, Yuanjie
Wei, Qingquan
Liu, Yong
Ren, Lufeng
Wang, Chenyu
Yu, Yude
One step DNA amplification of mammalian cells in picoliter microwell arrays
title One step DNA amplification of mammalian cells in picoliter microwell arrays
title_full One step DNA amplification of mammalian cells in picoliter microwell arrays
title_fullStr One step DNA amplification of mammalian cells in picoliter microwell arrays
title_full_unstemmed One step DNA amplification of mammalian cells in picoliter microwell arrays
title_short One step DNA amplification of mammalian cells in picoliter microwell arrays
title_sort one step dna amplification of mammalian cells in picoliter microwell arrays
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059946/
https://www.ncbi.nlm.nih.gov/pubmed/35520517
http://dx.doi.org/10.1039/c8ra06717a
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