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Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model

INTRODUCTION: Osteomyelitis is characterized by intensive inflammatory bone disease and remains a clinical challenge in orthopedic surgery, despite the advances made in medical and surgical therapies. Staphylococcus aureusis a major causative agent of osteomyelitis, causing the progressive inflammat...

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Autores principales: Wu, Wen-Jiao, Xia, Chang-Liang, Ou, Shuan-Ji, Yang, Yang, Zhou, Xiao-Zhong, Ma, Yun-Fei, Hou, Yi-Long, Wang, Fa-Zheng, Yang, Qing-Po, Qi, Yong, Xu, Chang-Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059993/
https://www.ncbi.nlm.nih.gov/pubmed/35509324
http://dx.doi.org/10.2147/JIR.S346627
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author Wu, Wen-Jiao
Xia, Chang-Liang
Ou, Shuan-Ji
Yang, Yang
Zhou, Xiao-Zhong
Ma, Yun-Fei
Hou, Yi-Long
Wang, Fa-Zheng
Yang, Qing-Po
Qi, Yong
Xu, Chang-Peng
author_facet Wu, Wen-Jiao
Xia, Chang-Liang
Ou, Shuan-Ji
Yang, Yang
Zhou, Xiao-Zhong
Ma, Yun-Fei
Hou, Yi-Long
Wang, Fa-Zheng
Yang, Qing-Po
Qi, Yong
Xu, Chang-Peng
author_sort Wu, Wen-Jiao
collection PubMed
description INTRODUCTION: Osteomyelitis is characterized by intensive inflammatory bone disease and remains a clinical challenge in orthopedic surgery, despite the advances made in medical and surgical therapies. Staphylococcus aureusis a major causative agent of osteomyelitis, causing the progressive inflammatory destruction of bone. Prophylaxis of osteomyelitis during orthopedic surgery is necessary. NFκB essential modulator–binding domain (NBD) peptides are cell-permeable peptide inhibitors of the IκB-kinase complex. The prophylactic effect of NBD peptides in relieving inflammation and inhibiting bone defects in osteomyelitis is still under investigation. Our purpose was to determine the preventive effect of NBD peptides in S. aureus infection–induced bone defects in osteomyelitis. METHODS: An S. aureus osteomyelitis rabbit model was used in this study. The rabbits were divided into four groups: NBD, cefazolin, control, and PBS. Clinical and laboratory indicators of erythrocyte-sedimentation rate, CRP, and TNFα levels were assessed to monitor systemic reactions. The efficacy of NBD peptides in S. aureus–induced osteomyelitis was evaluated by radiological, histological, and microbiological examinations, immunohistochemistry, immunofluorescence, and micro-CT scans. RESULTS: In general, NBD peptides effectively reduced clinical signs in rabbits when compared with the control group. Radiography indicated that there was more severe osteomyelitis in the bacterium-infection control group. There was no significance between cefazolin- and NBD-group average scores. The histological results of the lesion slices further confirmed different severity among the groups. Additionally, significant pathological differences were found between the cefazolin and NBD groups, and the PBS group showed no obvious pathological changes. CONCLUSION: Prophylactic administration of NBD peptides to bone-defect areas inhibited bacterial spread and promoted bone regeneration, making NBD peptides a possible treatment option for prophylaxis in bone infections.
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spelling pubmed-90599932022-05-03 Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model Wu, Wen-Jiao Xia, Chang-Liang Ou, Shuan-Ji Yang, Yang Zhou, Xiao-Zhong Ma, Yun-Fei Hou, Yi-Long Wang, Fa-Zheng Yang, Qing-Po Qi, Yong Xu, Chang-Peng J Inflamm Res Original Research INTRODUCTION: Osteomyelitis is characterized by intensive inflammatory bone disease and remains a clinical challenge in orthopedic surgery, despite the advances made in medical and surgical therapies. Staphylococcus aureusis a major causative agent of osteomyelitis, causing the progressive inflammatory destruction of bone. Prophylaxis of osteomyelitis during orthopedic surgery is necessary. NFκB essential modulator–binding domain (NBD) peptides are cell-permeable peptide inhibitors of the IκB-kinase complex. The prophylactic effect of NBD peptides in relieving inflammation and inhibiting bone defects in osteomyelitis is still under investigation. Our purpose was to determine the preventive effect of NBD peptides in S. aureus infection–induced bone defects in osteomyelitis. METHODS: An S. aureus osteomyelitis rabbit model was used in this study. The rabbits were divided into four groups: NBD, cefazolin, control, and PBS. Clinical and laboratory indicators of erythrocyte-sedimentation rate, CRP, and TNFα levels were assessed to monitor systemic reactions. The efficacy of NBD peptides in S. aureus–induced osteomyelitis was evaluated by radiological, histological, and microbiological examinations, immunohistochemistry, immunofluorescence, and micro-CT scans. RESULTS: In general, NBD peptides effectively reduced clinical signs in rabbits when compared with the control group. Radiography indicated that there was more severe osteomyelitis in the bacterium-infection control group. There was no significance between cefazolin- and NBD-group average scores. The histological results of the lesion slices further confirmed different severity among the groups. Additionally, significant pathological differences were found between the cefazolin and NBD groups, and the PBS group showed no obvious pathological changes. CONCLUSION: Prophylactic administration of NBD peptides to bone-defect areas inhibited bacterial spread and promoted bone regeneration, making NBD peptides a possible treatment option for prophylaxis in bone infections. Dove 2022-04-28 /pmc/articles/PMC9059993/ /pubmed/35509324 http://dx.doi.org/10.2147/JIR.S346627 Text en © 2022 Wu et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wu, Wen-Jiao
Xia, Chang-Liang
Ou, Shuan-Ji
Yang, Yang
Zhou, Xiao-Zhong
Ma, Yun-Fei
Hou, Yi-Long
Wang, Fa-Zheng
Yang, Qing-Po
Qi, Yong
Xu, Chang-Peng
Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title_full Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title_fullStr Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title_full_unstemmed Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title_short Prophylactic Effects of NFκB Essential Modulator–Binding Domain Peptides on Bone Infection: An Experimental Study in a Rabbit Model
title_sort prophylactic effects of nfκb essential modulator–binding domain peptides on bone infection: an experimental study in a rabbit model
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059993/
https://www.ncbi.nlm.nih.gov/pubmed/35509324
http://dx.doi.org/10.2147/JIR.S346627
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