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Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters

Ultrasound devices are common in muscle physiology laboratories due to their ease of use and validity to assess skeletal muscle characteristics. The current study assessed the reliability of ultrasound skeletal muscle image analysis across multiple raters with limited experience. Vastus lateralis (V...

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Autores principales: Cleary, Christopher J., Nabavizadeh, Omid, Young, Kaycie L., Herda, Ashley A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060372/
https://www.ncbi.nlm.nih.gov/pubmed/35500010
http://dx.doi.org/10.1371/journal.pone.0267641
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author Cleary, Christopher J.
Nabavizadeh, Omid
Young, Kaycie L.
Herda, Ashley A.
author_facet Cleary, Christopher J.
Nabavizadeh, Omid
Young, Kaycie L.
Herda, Ashley A.
author_sort Cleary, Christopher J.
collection PubMed
description Ultrasound devices are common in muscle physiology laboratories due to their ease of use and validity to assess skeletal muscle characteristics. The current study assessed the reliability of ultrasound skeletal muscle image analysis across multiple raters with limited experience. Vastus lateralis (VL), rectus femoris (RF), and first dorsal interosseus (FDI) images were separately analyzed by three novice raters to determine muscle thickness (MT), cross-sectional area (CSA), and echo-intensity (EI). Separate analyses of variance (ANOVA) assessed statistical differences between and within raters. Intra-class correlation coefficients (ICC) between (inter-rater) and within (intra-rater) raters, the standard error of the measurement (SEM) and minimal difference needed to be considered real were calculated. Inter-rater reliability was high for the VL and RF (ICC: 0.984–0.999), while the FDI was lower (0.614–0.962). Further, intra-rater reliability was greater than 0.961 for each rater. SEM values calculated for inter-rater reliability expressed as a percentage of the mean ranged from 0.4–5.8% across variables. Similarly, SEM values for intra-rater reliability were between 0.8–5.8%, 0.6–3.6%, and 0.4–3.2% for Raters 1, 2 and 3, respectively. Despite this, significant differences (p<0.05) between raters were observed for RF MT and EI, VL CSA and EI, and FDI MT, suggesting that potentially more measurement trials or greater practice time may be necessary to reduce systematic error among multiple raters. Post-image acquisition processing is reliable among and within raters as determined through ICCs and SEMs. This study provided consistent results among three separate novice raters given the same training, a unique yet realistic setting in muscle physiology laboratories.
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spelling pubmed-90603722022-05-03 Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters Cleary, Christopher J. Nabavizadeh, Omid Young, Kaycie L. Herda, Ashley A. PLoS One Research Article Ultrasound devices are common in muscle physiology laboratories due to their ease of use and validity to assess skeletal muscle characteristics. The current study assessed the reliability of ultrasound skeletal muscle image analysis across multiple raters with limited experience. Vastus lateralis (VL), rectus femoris (RF), and first dorsal interosseus (FDI) images were separately analyzed by three novice raters to determine muscle thickness (MT), cross-sectional area (CSA), and echo-intensity (EI). Separate analyses of variance (ANOVA) assessed statistical differences between and within raters. Intra-class correlation coefficients (ICC) between (inter-rater) and within (intra-rater) raters, the standard error of the measurement (SEM) and minimal difference needed to be considered real were calculated. Inter-rater reliability was high for the VL and RF (ICC: 0.984–0.999), while the FDI was lower (0.614–0.962). Further, intra-rater reliability was greater than 0.961 for each rater. SEM values calculated for inter-rater reliability expressed as a percentage of the mean ranged from 0.4–5.8% across variables. Similarly, SEM values for intra-rater reliability were between 0.8–5.8%, 0.6–3.6%, and 0.4–3.2% for Raters 1, 2 and 3, respectively. Despite this, significant differences (p<0.05) between raters were observed for RF MT and EI, VL CSA and EI, and FDI MT, suggesting that potentially more measurement trials or greater practice time may be necessary to reduce systematic error among multiple raters. Post-image acquisition processing is reliable among and within raters as determined through ICCs and SEMs. This study provided consistent results among three separate novice raters given the same training, a unique yet realistic setting in muscle physiology laboratories. Public Library of Science 2022-05-02 /pmc/articles/PMC9060372/ /pubmed/35500010 http://dx.doi.org/10.1371/journal.pone.0267641 Text en © 2022 Cleary et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cleary, Christopher J.
Nabavizadeh, Omid
Young, Kaycie L.
Herda, Ashley A.
Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title_full Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title_fullStr Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title_full_unstemmed Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title_short Skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
title_sort skeletal muscle analysis of panoramic ultrasound is reliable across multiple raters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060372/
https://www.ncbi.nlm.nih.gov/pubmed/35500010
http://dx.doi.org/10.1371/journal.pone.0267641
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