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Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications

The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a bioc...

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Autores principales: Dattola, Elisabetta, Parrotta, Elvira Immacolata, Scalise, Stefania, Perozziello, Gerardo, Limongi, Tania, Candeloro, Patrizio, Coluccio, Maria Laura, Maletta, Carmine, Bruno, Luigi, De Angelis, Maria Teresa, Santamaria, Gianluca, Mollace, Vincenzo, Lamanna, Ernesto, Di Fabrizio, Enzo, Cuda, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060459/
https://www.ncbi.nlm.nih.gov/pubmed/35520194
http://dx.doi.org/10.1039/c8ra08187e
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author Dattola, Elisabetta
Parrotta, Elvira Immacolata
Scalise, Stefania
Perozziello, Gerardo
Limongi, Tania
Candeloro, Patrizio
Coluccio, Maria Laura
Maletta, Carmine
Bruno, Luigi
De Angelis, Maria Teresa
Santamaria, Gianluca
Mollace, Vincenzo
Lamanna, Ernesto
Di Fabrizio, Enzo
Cuda, Giovanni
author_facet Dattola, Elisabetta
Parrotta, Elvira Immacolata
Scalise, Stefania
Perozziello, Gerardo
Limongi, Tania
Candeloro, Patrizio
Coluccio, Maria Laura
Maletta, Carmine
Bruno, Luigi
De Angelis, Maria Teresa
Santamaria, Gianluca
Mollace, Vincenzo
Lamanna, Ernesto
Di Fabrizio, Enzo
Cuda, Giovanni
author_sort Dattola, Elisabetta
collection PubMed
description The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.
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spelling pubmed-90604592022-05-04 Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications Dattola, Elisabetta Parrotta, Elvira Immacolata Scalise, Stefania Perozziello, Gerardo Limongi, Tania Candeloro, Patrizio Coluccio, Maria Laura Maletta, Carmine Bruno, Luigi De Angelis, Maria Teresa Santamaria, Gianluca Mollace, Vincenzo Lamanna, Ernesto Di Fabrizio, Enzo Cuda, Giovanni RSC Adv Chemistry The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications. The Royal Society of Chemistry 2019-02-14 /pmc/articles/PMC9060459/ /pubmed/35520194 http://dx.doi.org/10.1039/c8ra08187e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Dattola, Elisabetta
Parrotta, Elvira Immacolata
Scalise, Stefania
Perozziello, Gerardo
Limongi, Tania
Candeloro, Patrizio
Coluccio, Maria Laura
Maletta, Carmine
Bruno, Luigi
De Angelis, Maria Teresa
Santamaria, Gianluca
Mollace, Vincenzo
Lamanna, Ernesto
Di Fabrizio, Enzo
Cuda, Giovanni
Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title_full Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title_fullStr Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title_full_unstemmed Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title_short Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications
title_sort development of 3d pva scaffolds for cardiac tissue engineering and cell screening applications
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060459/
https://www.ncbi.nlm.nih.gov/pubmed/35520194
http://dx.doi.org/10.1039/c8ra08187e
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