Drosophila females have an acoustic preference for symmetric males

In many species, including humans and Drosophila, symmetric individuals secure more matings, suggesting that bilateral symmetry signals the quality of potential mates and is subject to sexual selection. However, this idea remains controversial, largely because obtaining conclusive experimental evidence has been hindered by confounding effects arising from the methods used to increase asymmetry in test subjects. Here, we show that altering gravity during development increases asymmetry in Drosophila melanogaster without a detrimental effect on survival, growth, and behavior. Testing males with altered-gravity–induced asymmetry in female mate-choice assays revealed symmetry-based discrimination of males via auditory cues. Females similarly discriminated against males with genetically induced asymmetry, suggesting that their preference for symmetry is not specific to altered gravity. By segmenting the male courtship song into left and right wing-generated song-bouts, we detected asymmetry in the courtship song of altered-gravity males with asymmetric wings that experienced rejection. Females experimentally evolved in the absence of mate choice lacked this preference for symmetry, suggesting that symmetry is maintained by sexual selection. Our data provide evidence for the role of symmetry in sexual selection and reveal how nonvisual cues can flag mate asymmetry during courtship.