Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound

Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the...

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Detalles Bibliográficos
Autores principales: Sakai, Rika, Iguchi, Hiroki, Maruyama, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060625/
https://www.ncbi.nlm.nih.gov/pubmed/35520182
http://dx.doi.org/10.1039/c8ra09421g
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author Sakai, Rika
Iguchi, Hiroki
Maruyama, Tatsuo
author_facet Sakai, Rika
Iguchi, Hiroki
Maruyama, Tatsuo
author_sort Sakai, Rika
collection PubMed
description Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer.
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spelling pubmed-90606252022-05-04 Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound Sakai, Rika Iguchi, Hiroki Maruyama, Tatsuo RSC Adv Chemistry Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer. The Royal Society of Chemistry 2019-02-18 /pmc/articles/PMC9060625/ /pubmed/35520182 http://dx.doi.org/10.1039/c8ra09421g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Sakai, Rika
Iguchi, Hiroki
Maruyama, Tatsuo
Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title_full Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title_fullStr Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title_full_unstemmed Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title_short Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
title_sort quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060625/
https://www.ncbi.nlm.nih.gov/pubmed/35520182
http://dx.doi.org/10.1039/c8ra09421g
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AT iguchihiroki quantificationofazidegroupsonamaterialsurfaceandabiomoleculeusingaclickableandcleavablefluorescentcompound
AT maruyamatatsuo quantificationofazidegroupsonamaterialsurfaceandabiomoleculeusingaclickableandcleavablefluorescentcompound

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