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Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes

Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer...

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Autores principales: Luan, Xingkun, Wang, Haozheng, Xiang, Zehong, Zhao, Jiruo, Feng, Ying, Shi, Qiang, Baijun liu, Gong, Yumei, Wong, Shing-Chung, Yin, Jinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060672/
https://www.ncbi.nlm.nih.gov/pubmed/35515950
http://dx.doi.org/10.1039/c8ra08215d
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author Luan, Xingkun
Wang, Haozheng
Xiang, Zehong
Zhao, Jiruo
Feng, Ying
Shi, Qiang
Baijun liu,
Gong, Yumei
Wong, Shing-Chung
Yin, Jinghua
author_facet Luan, Xingkun
Wang, Haozheng
Xiang, Zehong
Zhao, Jiruo
Feng, Ying
Shi, Qiang
Baijun liu,
Gong, Yumei
Wong, Shing-Chung
Yin, Jinghua
author_sort Luan, Xingkun
collection PubMed
description Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer, we fabricated 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G)-loaded polycaprolactone (PCL)-crown ether micro/nanofibers on the surface of styrene-b-(ethylene-co-butylene)-b-styrene (SEBS). Our strategy is based on the sensitive response of the crown ether to leaked potassium, causing the release of AA-2G, the AA-2G can then remove the excess ROS, maintaining the Na/K-pump activity and the cell integrity. We demonstrated that the PCL-crown ether micro/nanofibers have been well prepared on the surface of SEBS; the micro/nanofibers provide a sensitive response to excess K(+) and trigger the rapid release of AA-2G. AA-2G then acts as an antioxidant to reduce the excess ROS and maintain the Na/K-pump activity to mitigate cation leaks, resulting in the reduced hemolysis of the preserved erythrocytes. Our work thus provides a novel method for the development of plasticizer-free polymers for the storage of erythrocytes, and has the potential to be used to fabricate long-term anti-hemolytic biomaterials for in vivo use.
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spelling pubmed-90606722022-05-04 Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes Luan, Xingkun Wang, Haozheng Xiang, Zehong Zhao, Jiruo Feng, Ying Shi, Qiang Baijun liu, Gong, Yumei Wong, Shing-Chung Yin, Jinghua RSC Adv Chemistry Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer, we fabricated 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G)-loaded polycaprolactone (PCL)-crown ether micro/nanofibers on the surface of styrene-b-(ethylene-co-butylene)-b-styrene (SEBS). Our strategy is based on the sensitive response of the crown ether to leaked potassium, causing the release of AA-2G, the AA-2G can then remove the excess ROS, maintaining the Na/K-pump activity and the cell integrity. We demonstrated that the PCL-crown ether micro/nanofibers have been well prepared on the surface of SEBS; the micro/nanofibers provide a sensitive response to excess K(+) and trigger the rapid release of AA-2G. AA-2G then acts as an antioxidant to reduce the excess ROS and maintain the Na/K-pump activity to mitigate cation leaks, resulting in the reduced hemolysis of the preserved erythrocytes. Our work thus provides a novel method for the development of plasticizer-free polymers for the storage of erythrocytes, and has the potential to be used to fabricate long-term anti-hemolytic biomaterials for in vivo use. The Royal Society of Chemistry 2019-02-11 /pmc/articles/PMC9060672/ /pubmed/35515950 http://dx.doi.org/10.1039/c8ra08215d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Luan, Xingkun
Wang, Haozheng
Xiang, Zehong
Zhao, Jiruo
Feng, Ying
Shi, Qiang
Baijun liu,
Gong, Yumei
Wong, Shing-Chung
Yin, Jinghua
Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title_full Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title_fullStr Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title_full_unstemmed Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title_short Construction of K(+) responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes
title_sort construction of k(+) responsive surface on sebs to reduce the hemolysis of preserved erythrocytes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060672/
https://www.ncbi.nlm.nih.gov/pubmed/35515950
http://dx.doi.org/10.1039/c8ra08215d
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