Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening

• The biosensor reported in our study can monitor SARS-CoV-2 M(pro) activity in living cells instead of in vitro solutions. • The biosensor reported in our study is sensitive and easy to operate. • It is suitable for high-throughput screening. • It has the potential to be used in small animal models...

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Autores principales: Zhang, Yuan, Li, Chunjie, Ke, Xianliang, Luo, Dan, Liu, Yan, Chen, Quanjiao, Wang, Hanzhong, Song, Xiaohui, Zheng, Zhenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060717/
https://www.ncbi.nlm.nih.gov/pubmed/35513272
http://dx.doi.org/10.1016/j.virs.2022.04.002
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author Zhang, Yuan
Li, Chunjie
Ke, Xianliang
Luo, Dan
Liu, Yan
Chen, Quanjiao
Wang, Hanzhong
Song, Xiaohui
Zheng, Zhenhua
author_facet Zhang, Yuan
Li, Chunjie
Ke, Xianliang
Luo, Dan
Liu, Yan
Chen, Quanjiao
Wang, Hanzhong
Song, Xiaohui
Zheng, Zhenhua
author_sort Zhang, Yuan
collection PubMed
description • The biosensor reported in our study can monitor SARS-CoV-2 M(pro) activity in living cells instead of in vitro solutions. • The biosensor reported in our study is sensitive and easy to operate. • It is suitable for high-throughput screening. • It has the potential to be used in small animal models.
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spelling pubmed-90607172022-05-03 Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening Zhang, Yuan Li, Chunjie Ke, Xianliang Luo, Dan Liu, Yan Chen, Quanjiao Wang, Hanzhong Song, Xiaohui Zheng, Zhenhua Virol Sin Letter • The biosensor reported in our study can monitor SARS-CoV-2 M(pro) activity in living cells instead of in vitro solutions. • The biosensor reported in our study is sensitive and easy to operate. • It is suitable for high-throughput screening. • It has the potential to be used in small animal models. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-05-02 /pmc/articles/PMC9060717/ /pubmed/35513272 http://dx.doi.org/10.1016/j.virs.2022.04.002 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Zhang, Yuan
Li, Chunjie
Ke, Xianliang
Luo, Dan
Liu, Yan
Chen, Quanjiao
Wang, Hanzhong
Song, Xiaohui
Zheng, Zhenhua
Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title_full Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title_fullStr Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title_full_unstemmed Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title_short Development of a biosensor assessing SARS-CoV-2 main protease proteolytic activity in living cells for antiviral drugs screening
title_sort development of a biosensor assessing sars-cov-2 main protease proteolytic activity in living cells for antiviral drugs screening
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060717/
https://www.ncbi.nlm.nih.gov/pubmed/35513272
http://dx.doi.org/10.1016/j.virs.2022.04.002
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