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Lipase-catalyzed synthesis of red pitaya (Hylocereus polyrhizus) seed oil esters for cosmeceutical applications: process optimization using response surface methodology

Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed usi...

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Detalles Bibliográficos
Autores principales: Abdullah, Asiah, Abd Gani, Siti Salwa, Yun Hin, Taufiq Yap, Haiyee, Zaibunnisa Abdul, Zaidan, Uswatun Hasanah, Kassim, Mohd Azlan, Effendi Halmi, Mohd Izuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060793/
https://www.ncbi.nlm.nih.gov/pubmed/35515910
http://dx.doi.org/10.1039/c8ra09418g
Descripción
Sumario:Esters were synthesized via the alcoholysis of red pitaya seed oil with oleyl alcohol catalyzed by immobilized lipase, Lipozyme RM IM. The effects of synthesis parameters, including temperature, time, substrate molar ratio and enzyme loading, on the yield and productivity of esters were assessed using a central composite response surface design. The optimum yield and productivity were predicted to be about 80.00% and 0.58 mmol h(−1), respectively, at a synthesis temperature of 50.5 °C, time of 4 h, substrate molar ratio of 3.4 : 1 and with 0.17 g of enzyme. Esters were synthesized under the optimum synthesis conditions; it was found that the average yield and productivity were 82.48 ± 4.57% and 0.62 ± 0.04 mmol h(−1), respectively, revealing good correspondence with the predicted values. The main esters were oleyl linoleate, oleyl oleate, oleyl palmitate and oleyl stearate. The synthesized esters exhibited no irritancy effects and their physicochemical properties showed their suitability for use as cosmeceutical ingredients.