Cargando…

Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier

Nowadays, owing to their large surface area, enhanced pore volume, increased porosity, and variable pore size, mesoporous materials, such as mesoporous silica and mesoporous carbon, have attracted significant attention in the areas of physical science and biomedical sciences. Due to their compositio...

Descripción completa

Detalles Bibliográficos
Autores principales: Lett, J. Anita, Sundareswari, M., Ravichandran, K., Latha, M. Bavani, Sagadevan, Suresh, Bin Johan, Mohd. Rafie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060915/
https://www.ncbi.nlm.nih.gov/pubmed/35517282
http://dx.doi.org/10.1039/c9ra00051h
_version_ 1784698610733023232
author Lett, J. Anita
Sundareswari, M.
Ravichandran, K.
Latha, M. Bavani
Sagadevan, Suresh
Bin Johan, Mohd. Rafie
author_facet Lett, J. Anita
Sundareswari, M.
Ravichandran, K.
Latha, M. Bavani
Sagadevan, Suresh
Bin Johan, Mohd. Rafie
author_sort Lett, J. Anita
collection PubMed
description Nowadays, owing to their large surface area, enhanced pore volume, increased porosity, and variable pore size, mesoporous materials, such as mesoporous silica and mesoporous carbon, have attracted significant attention in the areas of physical science and biomedical sciences. Due to their compositional and biological similarities to natural tissues, synthetic nanoscaled mesoporous hydroxyapatite (MPHA) nanoparticles possess good biocompatibility, bioactivity, and osteoconductivity and have recently expanded their applicability in a wide range of fields such as in tissue replacement, drug/gene delivery carriers, and biocompatible coatings. In this study, we defined a novel route to synthesize mesoporous hydroxyapatite nanoparticles by the sol–gel method in the presence of stearic acid (SA), a biocompatible medium chain fatty acid that would function as an organic modifier. The as-prepared HAP particles were subjected to structural, functional and morphological characterization. Mesopores in HAP were observed for samples synthesized at pH 11 by removing the organic template. The porosity of HAP was confirmed by the Brunauer–Emmett–Teller (BET) analysis. The apatite deposition phenomenon in simulated body fluid at pH 7.4 confirms their in vitro bioactivity, and based on the in vitro cytotoxicity examined using Vero cell cultures, the as-prepared HAP particles exhibit excellent cytocompatibility and cell viability as high as 83% at an extract concentration as low as 25%. Moreover, the loading and leaching behavior of the drug in mesoporous HAP was studied using methionine (MT), an essential amino acid. These results confirm that nano mesoporous hydroxyapatite loaded with MT can be a potential aspirant as a biomaterial in biomedical applications.
format Online
Article
Text
id pubmed-9060915
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90609152022-05-04 Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier Lett, J. Anita Sundareswari, M. Ravichandran, K. Latha, M. Bavani Sagadevan, Suresh Bin Johan, Mohd. Rafie RSC Adv Chemistry Nowadays, owing to their large surface area, enhanced pore volume, increased porosity, and variable pore size, mesoporous materials, such as mesoporous silica and mesoporous carbon, have attracted significant attention in the areas of physical science and biomedical sciences. Due to their compositional and biological similarities to natural tissues, synthetic nanoscaled mesoporous hydroxyapatite (MPHA) nanoparticles possess good biocompatibility, bioactivity, and osteoconductivity and have recently expanded their applicability in a wide range of fields such as in tissue replacement, drug/gene delivery carriers, and biocompatible coatings. In this study, we defined a novel route to synthesize mesoporous hydroxyapatite nanoparticles by the sol–gel method in the presence of stearic acid (SA), a biocompatible medium chain fatty acid that would function as an organic modifier. The as-prepared HAP particles were subjected to structural, functional and morphological characterization. Mesopores in HAP were observed for samples synthesized at pH 11 by removing the organic template. The porosity of HAP was confirmed by the Brunauer–Emmett–Teller (BET) analysis. The apatite deposition phenomenon in simulated body fluid at pH 7.4 confirms their in vitro bioactivity, and based on the in vitro cytotoxicity examined using Vero cell cultures, the as-prepared HAP particles exhibit excellent cytocompatibility and cell viability as high as 83% at an extract concentration as low as 25%. Moreover, the loading and leaching behavior of the drug in mesoporous HAP was studied using methionine (MT), an essential amino acid. These results confirm that nano mesoporous hydroxyapatite loaded with MT can be a potential aspirant as a biomaterial in biomedical applications. The Royal Society of Chemistry 2019-02-20 /pmc/articles/PMC9060915/ /pubmed/35517282 http://dx.doi.org/10.1039/c9ra00051h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Lett, J. Anita
Sundareswari, M.
Ravichandran, K.
Latha, M. Bavani
Sagadevan, Suresh
Bin Johan, Mohd. Rafie
Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title_full Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title_fullStr Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title_full_unstemmed Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title_short Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
title_sort tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060915/
https://www.ncbi.nlm.nih.gov/pubmed/35517282
http://dx.doi.org/10.1039/c9ra00051h
work_keys_str_mv AT lettjanita tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier
AT sundareswarim tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier
AT ravichandrank tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier
AT lathambavani tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier
AT sagadevansuresh tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier
AT binjohanmohdrafie tailoringthemorphologicalfeaturesofsolgelsynthesizedmesoporoushydroxyapatiteusingfattyacidsasanorganicmodifier