Cargando…
RNA ligation of very small pseudo nick structures by T4 RNA ligase 2, leading to efficient production of versatile RNA rings
T4 RNA ligase 2 catalyses two types of reactions: (i) sealing of a nick structure in double-stranded RNA and (ii) connection of two single-stranded RNA strands. In order to obtain comprehensive views on these two types of reactions and widen the application scope of this RNA ligase, we here systemat...
Autores principales: | Cheng, Kai, An, Ran, Cui, Yixiao, Zhang, Yaping, Han, Xutiange, Sui, Zhe, Chen, Hui, Liang, Xingguo, Komiyama, Makoto |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061723/ https://www.ncbi.nlm.nih.gov/pubmed/35518706 http://dx.doi.org/10.1039/c9ra01513b |
Ejemplares similares
-
Terminal hairpin in oligonucleotide dominantly prioritizes intramolecular cyclization by T4 ligase over intermolecular polymerization: an exclusive methodology for producing ssDNA rings
por: Cui, Yixiao, et al.
Publicado: (2018) -
Cyclization of secondarily structured oligonucleotides to single-stranded rings by using Taq DNA ligase at high temperatures
por: Cui, Yixiao, et al.
Publicado: (2018) -
Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand
por: Chen, Hui, et al.
Publicado: (2020) -
Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi
por: Unciuleac, Mihaela-Carmen, et al.
Publicado: (2015) -
Deinococcus radiodurans RNA ligase exemplifies a novel ligase clade with a distinctive N-terminal module that is important for 5′-PO(4) nick sealing and ligase adenylylation but dispensable for phosphodiester formation at an adenylylated nick
por: Raymond, Amy, et al.
Publicado: (2007)