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Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing
The differences in size and function between primate and rodent brains, and the association of disturbed excitatory/inhibitory balance with many neurodevelopmental disorders highlight the importance to study primate ganglionic eminences (GEs) development. Here we used single-cell RNA and ATAC sequen...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061815/ https://www.ncbi.nlm.nih.gov/pubmed/35273378 http://dx.doi.org/10.1038/s41422-022-00635-9 |
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author | Zhao, Ziqi Zhang, Dan Yang, Fuqiang Xu, Mingrui Zhao, Shaoli Pan, Taotao Liu, Chuanyu Liu, Yongjie Wu, Qingfeng Tu, Qiang Zhou, Ping Li, Rong Kang, Jia Zhu, Lan Gao, Fei Wang, Yaqing Xu, Zhiheng |
author_facet | Zhao, Ziqi Zhang, Dan Yang, Fuqiang Xu, Mingrui Zhao, Shaoli Pan, Taotao Liu, Chuanyu Liu, Yongjie Wu, Qingfeng Tu, Qiang Zhou, Ping Li, Rong Kang, Jia Zhu, Lan Gao, Fei Wang, Yaqing Xu, Zhiheng |
author_sort | Zhao, Ziqi |
collection | PubMed |
description | The differences in size and function between primate and rodent brains, and the association of disturbed excitatory/inhibitory balance with many neurodevelopmental disorders highlight the importance to study primate ganglionic eminences (GEs) development. Here we used single-cell RNA and ATAC sequencing to characterize the emergence of cell diversity in monkey and human GEs where most striatal and cortical interneurons are generated. We identified regional and temporal diversity among progenitor cells which give rise to a variety of interneurons. These cells are specified within the primate GEs by well conserved gene regulatory networks, similar to those identified in mice. However, we detected, in human, several novel regulatory pathways or factors involved in the specification and migration of interneurons. Importantly, comparison of progenitors between our human and published mouse GE datasets led to the discovery and confirmation of outer radial glial cells in GEs in human cortex. Our findings reveal both evolutionarily conservative and nonconservative regulatory networks in primate GEs, which may contribute to their larger brain sizes and more complex neural networks compared with mouse. |
format | Online Article Text |
id | pubmed-9061815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-90618152022-05-04 Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing Zhao, Ziqi Zhang, Dan Yang, Fuqiang Xu, Mingrui Zhao, Shaoli Pan, Taotao Liu, Chuanyu Liu, Yongjie Wu, Qingfeng Tu, Qiang Zhou, Ping Li, Rong Kang, Jia Zhu, Lan Gao, Fei Wang, Yaqing Xu, Zhiheng Cell Res Article The differences in size and function between primate and rodent brains, and the association of disturbed excitatory/inhibitory balance with many neurodevelopmental disorders highlight the importance to study primate ganglionic eminences (GEs) development. Here we used single-cell RNA and ATAC sequencing to characterize the emergence of cell diversity in monkey and human GEs where most striatal and cortical interneurons are generated. We identified regional and temporal diversity among progenitor cells which give rise to a variety of interneurons. These cells are specified within the primate GEs by well conserved gene regulatory networks, similar to those identified in mice. However, we detected, in human, several novel regulatory pathways or factors involved in the specification and migration of interneurons. Importantly, comparison of progenitors between our human and published mouse GE datasets led to the discovery and confirmation of outer radial glial cells in GEs in human cortex. Our findings reveal both evolutionarily conservative and nonconservative regulatory networks in primate GEs, which may contribute to their larger brain sizes and more complex neural networks compared with mouse. Springer Nature Singapore 2022-03-10 2022-05 /pmc/articles/PMC9061815/ /pubmed/35273378 http://dx.doi.org/10.1038/s41422-022-00635-9 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhao, Ziqi Zhang, Dan Yang, Fuqiang Xu, Mingrui Zhao, Shaoli Pan, Taotao Liu, Chuanyu Liu, Yongjie Wu, Qingfeng Tu, Qiang Zhou, Ping Li, Rong Kang, Jia Zhu, Lan Gao, Fei Wang, Yaqing Xu, Zhiheng Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title_full | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title_fullStr | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title_full_unstemmed | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title_short | Evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell RNA and ATAC sequencing |
title_sort | evolutionarily conservative and non-conservative regulatory networks during primate interneuron development revealed by single-cell rna and atac sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061815/ https://www.ncbi.nlm.nih.gov/pubmed/35273378 http://dx.doi.org/10.1038/s41422-022-00635-9 |
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