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The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei

Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3...

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Autores principales: Dinh, Hoan X., Singh, Davinder, Gomez de la Cruz, Diana, Hensel, Goetz, Kumlehn, Jochen, Mascher, Martin, Stein, Nils, Perovic, Dragan, Ayliffe, Michael, Moscou, Matthew J., Park, Robert F., Pourkheirandish, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061838/
https://www.ncbi.nlm.nih.gov/pubmed/35501307
http://dx.doi.org/10.1038/s41467-022-29840-1
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author Dinh, Hoan X.
Singh, Davinder
Gomez de la Cruz, Diana
Hensel, Goetz
Kumlehn, Jochen
Mascher, Martin
Stein, Nils
Perovic, Dragan
Ayliffe, Michael
Moscou, Matthew J.
Park, Robert F.
Pourkheirandish, Mohammad
author_facet Dinh, Hoan X.
Singh, Davinder
Gomez de la Cruz, Diana
Hensel, Goetz
Kumlehn, Jochen
Mascher, Martin
Stein, Nils
Perovic, Dragan
Ayliffe, Michael
Moscou, Matthew J.
Park, Robert F.
Pourkheirandish, Mohammad
author_sort Dinh, Hoan X.
collection PubMed
description Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique predicted transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. The Rph3 gene was expressed only in interactions with Rph3-avirulent P. hordei isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like known transmembrane executors such as Bs3 and Xa7, heterologous expression of Rph3 in N. benthamiana induced a cell death response. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots.
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spelling pubmed-90618382022-05-04 The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei Dinh, Hoan X. Singh, Davinder Gomez de la Cruz, Diana Hensel, Goetz Kumlehn, Jochen Mascher, Martin Stein, Nils Perovic, Dragan Ayliffe, Michael Moscou, Matthew J. Park, Robert F. Pourkheirandish, Mohammad Nat Commun Article Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique predicted transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. The Rph3 gene was expressed only in interactions with Rph3-avirulent P. hordei isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like known transmembrane executors such as Bs3 and Xa7, heterologous expression of Rph3 in N. benthamiana induced a cell death response. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots. Nature Publishing Group UK 2022-05-02 /pmc/articles/PMC9061838/ /pubmed/35501307 http://dx.doi.org/10.1038/s41467-022-29840-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dinh, Hoan X.
Singh, Davinder
Gomez de la Cruz, Diana
Hensel, Goetz
Kumlehn, Jochen
Mascher, Martin
Stein, Nils
Perovic, Dragan
Ayliffe, Michael
Moscou, Matthew J.
Park, Robert F.
Pourkheirandish, Mohammad
The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title_full The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title_fullStr The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title_full_unstemmed The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title_short The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei
title_sort barley leaf rust resistance gene rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of puccinia hordei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061838/
https://www.ncbi.nlm.nih.gov/pubmed/35501307
http://dx.doi.org/10.1038/s41467-022-29840-1
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