Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin

Thyroid hormones are essential regulators of metabolism, development, and growth. They are formed from pairs of iodinated tyrosine residues within the precursor thyroglobulin (TG), a 660-kDa homodimer of the thyroid gland, by an oxidative coupling reaction. Tyrosine pairs that give rise to thyroid h...

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Autores principales: Marechal, Nils, Serrano, Banyuhay P., Zhang, Xinyan, Weitz, Charles J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061844/
https://www.ncbi.nlm.nih.gov/pubmed/35501346
http://dx.doi.org/10.1038/s41467-022-30082-4
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author Marechal, Nils
Serrano, Banyuhay P.
Zhang, Xinyan
Weitz, Charles J.
author_facet Marechal, Nils
Serrano, Banyuhay P.
Zhang, Xinyan
Weitz, Charles J.
author_sort Marechal, Nils
collection PubMed
description Thyroid hormones are essential regulators of metabolism, development, and growth. They are formed from pairs of iodinated tyrosine residues within the precursor thyroglobulin (TG), a 660-kDa homodimer of the thyroid gland, by an oxidative coupling reaction. Tyrosine pairs that give rise to thyroid hormones have been assigned within the structure of human TG, but the process of hormone formation is poorly understood. Here we report a ~3.3-Å cryo-EM structure of native bovine TG with nascent thyroid hormone formed at one of the predicted hormonogenic sites. Local structural rearrangements provide insight into mechanisms underlying thyroid hormone formation and stabilization.
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spelling pubmed-90618442022-05-04 Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin Marechal, Nils Serrano, Banyuhay P. Zhang, Xinyan Weitz, Charles J. Nat Commun Article Thyroid hormones are essential regulators of metabolism, development, and growth. They are formed from pairs of iodinated tyrosine residues within the precursor thyroglobulin (TG), a 660-kDa homodimer of the thyroid gland, by an oxidative coupling reaction. Tyrosine pairs that give rise to thyroid hormones have been assigned within the structure of human TG, but the process of hormone formation is poorly understood. Here we report a ~3.3-Å cryo-EM structure of native bovine TG with nascent thyroid hormone formed at one of the predicted hormonogenic sites. Local structural rearrangements provide insight into mechanisms underlying thyroid hormone formation and stabilization. Nature Publishing Group UK 2022-05-02 /pmc/articles/PMC9061844/ /pubmed/35501346 http://dx.doi.org/10.1038/s41467-022-30082-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Marechal, Nils
Serrano, Banyuhay P.
Zhang, Xinyan
Weitz, Charles J.
Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title_full Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title_fullStr Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title_full_unstemmed Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title_short Formation of thyroid hormone revealed by a cryo-EM structure of native bovine thyroglobulin
title_sort formation of thyroid hormone revealed by a cryo-em structure of native bovine thyroglobulin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061844/
https://www.ncbi.nlm.nih.gov/pubmed/35501346
http://dx.doi.org/10.1038/s41467-022-30082-4
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