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N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle

The fatty acid dehydrogenase fat-1 gene, derived from Caenorhabditis elegans, encodes n-3 polyunsaturated fatty acid dehydrogenase (Δ15 desaturase) and catalyzes the 18–20-carbon n-6 polyunsaturated fatty acids (n-6 PUFA) to generate corresponding n-3 polyunsaturated fatty acids (n-3 PUFA). Subseque...

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Autores principales: Wang, Xueqiao, Zhu, Lin, Wei, Zhuying, Gu, Mingjuan, Yang, Miaomiao, Zhou, Xinyu, Bai, Chunling, Su, Guanghua, Liu, Xuefei, Yang, Lei, Li, Guangpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061993/
https://www.ncbi.nlm.nih.gov/pubmed/35517863
http://dx.doi.org/10.3389/fmolb.2022.857491
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author Wang, Xueqiao
Zhu, Lin
Wei, Zhuying
Gu, Mingjuan
Yang, Miaomiao
Zhou, Xinyu
Bai, Chunling
Su, Guanghua
Liu, Xuefei
Yang, Lei
Li, Guangpeng
author_facet Wang, Xueqiao
Zhu, Lin
Wei, Zhuying
Gu, Mingjuan
Yang, Miaomiao
Zhou, Xinyu
Bai, Chunling
Su, Guanghua
Liu, Xuefei
Yang, Lei
Li, Guangpeng
author_sort Wang, Xueqiao
collection PubMed
description The fatty acid dehydrogenase fat-1 gene, derived from Caenorhabditis elegans, encodes n-3 polyunsaturated fatty acid dehydrogenase (Δ15 desaturase) and catalyzes the 18–20-carbon n-6 polyunsaturated fatty acids (n-6 PUFA) to generate corresponding n-3 polyunsaturated fatty acids (n-3 PUFA). Subsequently, fat-1 can influence the n-6: n-3 PUFA ratio in fat-1 transgenic cells. This study aimed to explore which processes of energy metabolism are affected exogenous fat-1 transgene and the relationship between these effects and DNA methylation. Compared with the wild-type group, the n-3 PUFA content in fat-1 transgenic bovine fetal fibroblasts was significantly increased, and the n-6 PUFA content and the n-6: n-3 PUFA ratio decreased. In the context of energy metabolism, the increase of exogenous fat-1 transgene decreased ATP synthesis by 39% and reduced the activity and expression of key rate-limiting enzymes in glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation, thus weakening the cells’ capacity for ATP production. DNA methylation sequencing indicated that this inhibition of gene expression may be due to altered DNA methylation that regulates cell energy metabolism. Exogenous fat-1 transgenic cells showed changes in the degree of methylation in the promoter region of genes related to energy metabolism rate-limiting enzymes. We suggest that alters the balance of n-6/n-3 PUFA could regulate altered DNA methylation that affect mitochondrial energy metabolism.
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spelling pubmed-90619932022-05-04 N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle Wang, Xueqiao Zhu, Lin Wei, Zhuying Gu, Mingjuan Yang, Miaomiao Zhou, Xinyu Bai, Chunling Su, Guanghua Liu, Xuefei Yang, Lei Li, Guangpeng Front Mol Biosci Molecular Biosciences The fatty acid dehydrogenase fat-1 gene, derived from Caenorhabditis elegans, encodes n-3 polyunsaturated fatty acid dehydrogenase (Δ15 desaturase) and catalyzes the 18–20-carbon n-6 polyunsaturated fatty acids (n-6 PUFA) to generate corresponding n-3 polyunsaturated fatty acids (n-3 PUFA). Subsequently, fat-1 can influence the n-6: n-3 PUFA ratio in fat-1 transgenic cells. This study aimed to explore which processes of energy metabolism are affected exogenous fat-1 transgene and the relationship between these effects and DNA methylation. Compared with the wild-type group, the n-3 PUFA content in fat-1 transgenic bovine fetal fibroblasts was significantly increased, and the n-6 PUFA content and the n-6: n-3 PUFA ratio decreased. In the context of energy metabolism, the increase of exogenous fat-1 transgene decreased ATP synthesis by 39% and reduced the activity and expression of key rate-limiting enzymes in glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation, thus weakening the cells’ capacity for ATP production. DNA methylation sequencing indicated that this inhibition of gene expression may be due to altered DNA methylation that regulates cell energy metabolism. Exogenous fat-1 transgenic cells showed changes in the degree of methylation in the promoter region of genes related to energy metabolism rate-limiting enzymes. We suggest that alters the balance of n-6/n-3 PUFA could regulate altered DNA methylation that affect mitochondrial energy metabolism. Frontiers Media S.A. 2022-04-19 /pmc/articles/PMC9061993/ /pubmed/35517863 http://dx.doi.org/10.3389/fmolb.2022.857491 Text en Copyright © 2022 Wang, Zhu, Wei, Gu, Yang, Zhou, Bai, Su, Liu, Yang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Wang, Xueqiao
Zhu, Lin
Wei, Zhuying
Gu, Mingjuan
Yang, Miaomiao
Zhou, Xinyu
Bai, Chunling
Su, Guanghua
Liu, Xuefei
Yang, Lei
Li, Guangpeng
N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title_full N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title_fullStr N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title_full_unstemmed N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title_short N-3 Polyunsaturated Fatty Acid Dehydrogenase Fat-1 Regulates Mitochondrial Energy Metabolism by Altering DNA Methylation in Isolated Cells of Transgenic Cattle
title_sort n-3 polyunsaturated fatty acid dehydrogenase fat-1 regulates mitochondrial energy metabolism by altering dna methylation in isolated cells of transgenic cattle
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9061993/
https://www.ncbi.nlm.nih.gov/pubmed/35517863
http://dx.doi.org/10.3389/fmolb.2022.857491
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