Cargando…
Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721
T-2 toxin, produced by Fusarium moulds, is a type A trichothecene mycotoxin which is known to inhibit protein synthesis and also reported to induce DNA lesions, potentially causing DNA fragmentation. T-2 toxin is a very potent cytotoxic toxin, which displays anti-tumor properties. Nevertheless, more...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062006/ https://www.ncbi.nlm.nih.gov/pubmed/35517673 http://dx.doi.org/10.1039/c8ra09967g |
_version_ | 1784698836190494720 |
---|---|
author | Zhu, Wenhe Liu, Lei Dong, Yuan Meng, Guixian Tang, Lu Li, Yan Cai, Jianhui Wang, Huiyan |
author_facet | Zhu, Wenhe Liu, Lei Dong, Yuan Meng, Guixian Tang, Lu Li, Yan Cai, Jianhui Wang, Huiyan |
author_sort | Zhu, Wenhe |
collection | PubMed |
description | T-2 toxin, produced by Fusarium moulds, is a type A trichothecene mycotoxin which is known to inhibit protein synthesis and also reported to induce DNA lesions, potentially causing DNA fragmentation. T-2 toxin is a very potent cytotoxic toxin, which displays anti-tumor properties. Nevertheless, more studies are still needed to explore its antitumor mechanisms as well as its clinical application in cancer treatment. Here, we report the identification and characterization of a T-2 toxin produced by a Fusarium poae isolated from Jilin, Northeast China. 17 strains of Fusarium poae were screened for T-2 toxin-production and one strain with the highest yield was selected further studies. T-2 toxin produced by the selected Fusarium poae was isolated and purified by HPLC. Anticancer properties of the purified T-2 toxin were evaluated with human hepatoma cell SMMC-7721. The purified T-2 toxin inhibits the proliferation of SMMC-7721 cells and induces cell apoptosis. The mitochondrial membrane potential decreased and the intracellular ROS was up-regulated after T-2 treatment of the cells. Further studies revealed that T-2 treatment activates the intrinsic mitochondrial and MAPKs pathway. Our data provide insight into the promising application of the T-2 toxin in cancer treatment. |
format | Online Article Text |
id | pubmed-9062006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90620062022-05-04 Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 Zhu, Wenhe Liu, Lei Dong, Yuan Meng, Guixian Tang, Lu Li, Yan Cai, Jianhui Wang, Huiyan RSC Adv Chemistry T-2 toxin, produced by Fusarium moulds, is a type A trichothecene mycotoxin which is known to inhibit protein synthesis and also reported to induce DNA lesions, potentially causing DNA fragmentation. T-2 toxin is a very potent cytotoxic toxin, which displays anti-tumor properties. Nevertheless, more studies are still needed to explore its antitumor mechanisms as well as its clinical application in cancer treatment. Here, we report the identification and characterization of a T-2 toxin produced by a Fusarium poae isolated from Jilin, Northeast China. 17 strains of Fusarium poae were screened for T-2 toxin-production and one strain with the highest yield was selected further studies. T-2 toxin produced by the selected Fusarium poae was isolated and purified by HPLC. Anticancer properties of the purified T-2 toxin were evaluated with human hepatoma cell SMMC-7721. The purified T-2 toxin inhibits the proliferation of SMMC-7721 cells and induces cell apoptosis. The mitochondrial membrane potential decreased and the intracellular ROS was up-regulated after T-2 treatment of the cells. Further studies revealed that T-2 treatment activates the intrinsic mitochondrial and MAPKs pathway. Our data provide insight into the promising application of the T-2 toxin in cancer treatment. The Royal Society of Chemistry 2019-03-21 /pmc/articles/PMC9062006/ /pubmed/35517673 http://dx.doi.org/10.1039/c8ra09967g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhu, Wenhe Liu, Lei Dong, Yuan Meng, Guixian Tang, Lu Li, Yan Cai, Jianhui Wang, Huiyan Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title | Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title_full | Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title_fullStr | Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title_full_unstemmed | Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title_short | Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721 |
title_sort | identification and characterization of a t-2 toxin-producing fusarium poae strain and the anti-tumor effect of the t-2 toxin on human hepatoma cell line smmc-7721 |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062006/ https://www.ncbi.nlm.nih.gov/pubmed/35517673 http://dx.doi.org/10.1039/c8ra09967g |
work_keys_str_mv | AT zhuwenhe identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT liulei identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT dongyuan identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT mengguixian identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT tanglu identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT liyan identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT caijianhui identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 AT wanghuiyan identificationandcharacterizationofat2toxinproducingfusariumpoaestrainandtheantitumoreffectofthet2toxinonhumanhepatomacelllinesmmc7721 |