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Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation

Morphogen-mediated signaling is critical for proper organ development and stem cell function, and well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, and ligand-binding cell-surface glypicans. Here, we show that in the developing Drosophila ovary, canonical Wnt...

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Autores principales: Chen, Ting-An, Lin, Kun-Yang, Yang, Shun-Min, Tseng, Chen-Yuan, Wang, Yu-Ting, Lin, Chi-Hung, Luo, Lichao, Cai, Yu, Hsu, Hwei-Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062081/
https://www.ncbi.nlm.nih.gov/pubmed/35517512
http://dx.doi.org/10.3389/fcell.2022.877047
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author Chen, Ting-An
Lin, Kun-Yang
Yang, Shun-Min
Tseng, Chen-Yuan
Wang, Yu-Ting
Lin, Chi-Hung
Luo, Lichao
Cai, Yu
Hsu, Hwei-Jan
author_facet Chen, Ting-An
Lin, Kun-Yang
Yang, Shun-Min
Tseng, Chen-Yuan
Wang, Yu-Ting
Lin, Chi-Hung
Luo, Lichao
Cai, Yu
Hsu, Hwei-Jan
author_sort Chen, Ting-An
collection PubMed
description Morphogen-mediated signaling is critical for proper organ development and stem cell function, and well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, and ligand-binding cell-surface glypicans. Here, we show that in the developing Drosophila ovary, canonical Wnt signaling promotes the formation of somatic escort cells (ECs) and their protrusions, which establish a physical permeability barrier to define morphogen territories for proper germ cell differentiation. The protrusions shield germ cells from Dpp and Wingless morphogens produced by the germline stem cell (GSC) niche and normally only received by GSCs. Genetic disruption of EC protrusions allows GSC progeny to also receive Dpp and Wingless, which subsequently disrupt germ cell differentiation. Our results reveal a role for canonical Wnt signaling in specifying the ovarian somatic cells necessary for germ cell differentiation. Additionally, we demonstrate the morphogen-limiting function of this physical permeability barrier, which may be a common mechanism in other organs across species.
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spelling pubmed-90620812022-05-04 Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation Chen, Ting-An Lin, Kun-Yang Yang, Shun-Min Tseng, Chen-Yuan Wang, Yu-Ting Lin, Chi-Hung Luo, Lichao Cai, Yu Hsu, Hwei-Jan Front Cell Dev Biol Cell and Developmental Biology Morphogen-mediated signaling is critical for proper organ development and stem cell function, and well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, and ligand-binding cell-surface glypicans. Here, we show that in the developing Drosophila ovary, canonical Wnt signaling promotes the formation of somatic escort cells (ECs) and their protrusions, which establish a physical permeability barrier to define morphogen territories for proper germ cell differentiation. The protrusions shield germ cells from Dpp and Wingless morphogens produced by the germline stem cell (GSC) niche and normally only received by GSCs. Genetic disruption of EC protrusions allows GSC progeny to also receive Dpp and Wingless, which subsequently disrupt germ cell differentiation. Our results reveal a role for canonical Wnt signaling in specifying the ovarian somatic cells necessary for germ cell differentiation. Additionally, we demonstrate the morphogen-limiting function of this physical permeability barrier, which may be a common mechanism in other organs across species. Frontiers Media S.A. 2022-04-19 /pmc/articles/PMC9062081/ /pubmed/35517512 http://dx.doi.org/10.3389/fcell.2022.877047 Text en Copyright © 2022 Chen, Lin, Yang, Tseng, Wang, Lin, Luo, Cai and Hsu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chen, Ting-An
Lin, Kun-Yang
Yang, Shun-Min
Tseng, Chen-Yuan
Wang, Yu-Ting
Lin, Chi-Hung
Luo, Lichao
Cai, Yu
Hsu, Hwei-Jan
Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title_full Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title_fullStr Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title_full_unstemmed Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title_short Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation
title_sort canonical wnt signaling promotes formation of somatic permeability barrier for proper germ cell differentiation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062081/
https://www.ncbi.nlm.nih.gov/pubmed/35517512
http://dx.doi.org/10.3389/fcell.2022.877047
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