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Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system

The RNA-guided CRISPR/Cas9 system could cleave double-stranded DNA at the on-target sites but also induce off-target mutations in unexpected genomic regions. The base-pairing interaction of sgRNA with off-target DNA was still not well understood and also lacked a direct cell-based assay. Herein we d...

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Detalles Bibliográficos
Autores principales:	Wang, Dan, Niu, Cuili, Han, Jingxin, Ma, Dejun, Xi, Zhen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2019
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062094/ https://www.ncbi.nlm.nih.gov/pubmed/35517679 http://dx.doi.org/10.1039/c8ra10017a

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