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The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report

In recent years, the role of gastric and duodenal microbiota has acquired increasing importance in the homeostasis of the host, although, to date, most evidence concern the faecal microbiota. Indeed, the gastric, and duodenal microbiota are challenging to study, due to gastric acid, bile, digestive...

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Autores principales: Filardo, Simone, Scalese, Giulia, Virili, Camilla, Pontone, Stefano, Di Pietro, Marisa, Covelli, Antonio, Bedetti, Giorgio, Marinelli, Paride, Bruno, Giovanni, Stramazzo, Ilaria, Centanni, Marco, Sessa, Rosa, Severi, Carola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062108/
https://www.ncbi.nlm.nih.gov/pubmed/35521214
http://dx.doi.org/10.3389/fcimb.2022.854904
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author Filardo, Simone
Scalese, Giulia
Virili, Camilla
Pontone, Stefano
Di Pietro, Marisa
Covelli, Antonio
Bedetti, Giorgio
Marinelli, Paride
Bruno, Giovanni
Stramazzo, Ilaria
Centanni, Marco
Sessa, Rosa
Severi, Carola
author_facet Filardo, Simone
Scalese, Giulia
Virili, Camilla
Pontone, Stefano
Di Pietro, Marisa
Covelli, Antonio
Bedetti, Giorgio
Marinelli, Paride
Bruno, Giovanni
Stramazzo, Ilaria
Centanni, Marco
Sessa, Rosa
Severi, Carola
author_sort Filardo, Simone
collection PubMed
description In recent years, the role of gastric and duodenal microbiota has acquired increasing importance in the homeostasis of the host, although, to date, most evidence concern the faecal microbiota. Indeed, the gastric, and duodenal microbiota are challenging to study, due to gastric acid, bile, digestive enzymes, and rapid transit time. Specifically, the gastric acid environment may influence their bacterial composition since the acid barrier protects against orally ingested microorganisms and leads to their inactivation before reaching the intestine. The aim of this study was to assess a correlation between intragastric pH and gastric as well as intestinal microbiota of patients with histologic gastric alterations. pH was measured in the gastric juice and the bacterial composition in gastric and duodenal biopsies and faecal samples, was investigated via 16s rRNA gene sequencing. The main result is the direct correlation of duodenal microbiota biodiversity, via alpha diversity measures, with intragastric pH values. In particular, patients with hypochlorhydria showed increased duodenal microbiota biodiversity, higher intragastric pH values being prevalent in patients with chronic atrophic gastritis. Lastly, the latter was also strongly associated to the presence of oral bacteria, like Rothia mucilaginosa, Streptococcus salivarius and Granulicatella adiacens, in the duodenal microbiota. In conclusions, our results suggest a low-acid gastric environment as a contributive factor for duodenal dysbiosis, potentially leading to the development of pathological conditions of the gastrointestinal tract.
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spelling pubmed-90621082022-05-04 The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report Filardo, Simone Scalese, Giulia Virili, Camilla Pontone, Stefano Di Pietro, Marisa Covelli, Antonio Bedetti, Giorgio Marinelli, Paride Bruno, Giovanni Stramazzo, Ilaria Centanni, Marco Sessa, Rosa Severi, Carola Front Cell Infect Microbiol Cellular and Infection Microbiology In recent years, the role of gastric and duodenal microbiota has acquired increasing importance in the homeostasis of the host, although, to date, most evidence concern the faecal microbiota. Indeed, the gastric, and duodenal microbiota are challenging to study, due to gastric acid, bile, digestive enzymes, and rapid transit time. Specifically, the gastric acid environment may influence their bacterial composition since the acid barrier protects against orally ingested microorganisms and leads to their inactivation before reaching the intestine. The aim of this study was to assess a correlation between intragastric pH and gastric as well as intestinal microbiota of patients with histologic gastric alterations. pH was measured in the gastric juice and the bacterial composition in gastric and duodenal biopsies and faecal samples, was investigated via 16s rRNA gene sequencing. The main result is the direct correlation of duodenal microbiota biodiversity, via alpha diversity measures, with intragastric pH values. In particular, patients with hypochlorhydria showed increased duodenal microbiota biodiversity, higher intragastric pH values being prevalent in patients with chronic atrophic gastritis. Lastly, the latter was also strongly associated to the presence of oral bacteria, like Rothia mucilaginosa, Streptococcus salivarius and Granulicatella adiacens, in the duodenal microbiota. In conclusions, our results suggest a low-acid gastric environment as a contributive factor for duodenal dysbiosis, potentially leading to the development of pathological conditions of the gastrointestinal tract. Frontiers Media S.A. 2022-04-19 /pmc/articles/PMC9062108/ /pubmed/35521214 http://dx.doi.org/10.3389/fcimb.2022.854904 Text en Copyright © 2022 Filardo, Scalese, Virili, Pontone, Di Pietro, Covelli, Bedetti, Marinelli, Bruno, Stramazzo, Centanni, Sessa and Severi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Filardo, Simone
Scalese, Giulia
Virili, Camilla
Pontone, Stefano
Di Pietro, Marisa
Covelli, Antonio
Bedetti, Giorgio
Marinelli, Paride
Bruno, Giovanni
Stramazzo, Ilaria
Centanni, Marco
Sessa, Rosa
Severi, Carola
The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title_full The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title_fullStr The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title_full_unstemmed The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title_short The Potential Role of Hypochlorhydria in the Development of Duodenal Dysbiosis: A Preliminary Report
title_sort potential role of hypochlorhydria in the development of duodenal dysbiosis: a preliminary report
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062108/
https://www.ncbi.nlm.nih.gov/pubmed/35521214
http://dx.doi.org/10.3389/fcimb.2022.854904
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