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Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network

Cadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresse...

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Autores principales: Wang, Pei-Long, Lei, Xiao-Jin, Wang, Yuan-Yuan, Liu, Bai-chao, Wang, Dan-ni, Liu, Zhong-Yuan, Gao, Cai-Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062237/
https://www.ncbi.nlm.nih.gov/pubmed/35519810
http://dx.doi.org/10.3389/fpls.2022.843725
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author Wang, Pei-Long
Lei, Xiao-Jin
Wang, Yuan-Yuan
Liu, Bai-chao
Wang, Dan-ni
Liu, Zhong-Yuan
Gao, Cai-Qiu
author_facet Wang, Pei-Long
Lei, Xiao-Jin
Wang, Yuan-Yuan
Liu, Bai-chao
Wang, Dan-ni
Liu, Zhong-Yuan
Gao, Cai-Qiu
author_sort Wang, Pei-Long
collection PubMed
description Cadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresses. However, no systematic study has investigated the molecular mechanism of Cd tolerance in T. hispida. In the study, RNA-seq technique was applied to analyze the transcriptomic changes in T. hispida treated with 150 μmol L(–1) CdCl(2) for 24, 48, and 72 h compared with control. In total, 72,764 unigenes exhibited similar sequences in the Non-redundant nucleic acid database (NR database), while 36.3% of all these unigenes may be new transcripts. In addition, 6,778, 8,282, and 8,601 DEGs were detected at 24, 48, and 72 h, respectively. Functional annotation analysis indicated that many genes may be involved in Cd stress response, including ion bonding, signal transduction, stress sensing, hormone responses and ROS metabolism. A ThUGT gene from the abscisic acid (ABA) signaling pathway can enhance Cd resistance ability of T. hispida by regulating the production of ROS under Cd stress and inhibit absorption of Cd. The new transcriptome resources and data that we present in this study for T. hispida may facilitate investigation of molecular mechanisms governing Cd resistance.
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spelling pubmed-90622372022-05-04 Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network Wang, Pei-Long Lei, Xiao-Jin Wang, Yuan-Yuan Liu, Bai-chao Wang, Dan-ni Liu, Zhong-Yuan Gao, Cai-Qiu Front Plant Sci Plant Science Cadmium (Cd) pollution is widely detected in soil and has been recognized as a major environmental problem. Tamarix hispida is a woody halophyte, which can form natural forest on the desert and soil with 0.5 to 1% salt content, making it an ideal plant for the research on response to abiotic stresses. However, no systematic study has investigated the molecular mechanism of Cd tolerance in T. hispida. In the study, RNA-seq technique was applied to analyze the transcriptomic changes in T. hispida treated with 150 μmol L(–1) CdCl(2) for 24, 48, and 72 h compared with control. In total, 72,764 unigenes exhibited similar sequences in the Non-redundant nucleic acid database (NR database), while 36.3% of all these unigenes may be new transcripts. In addition, 6,778, 8,282, and 8,601 DEGs were detected at 24, 48, and 72 h, respectively. Functional annotation analysis indicated that many genes may be involved in Cd stress response, including ion bonding, signal transduction, stress sensing, hormone responses and ROS metabolism. A ThUGT gene from the abscisic acid (ABA) signaling pathway can enhance Cd resistance ability of T. hispida by regulating the production of ROS under Cd stress and inhibit absorption of Cd. The new transcriptome resources and data that we present in this study for T. hispida may facilitate investigation of molecular mechanisms governing Cd resistance. Frontiers Media S.A. 2022-04-18 /pmc/articles/PMC9062237/ /pubmed/35519810 http://dx.doi.org/10.3389/fpls.2022.843725 Text en Copyright © 2022 Wang, Lei, Wang, Liu, Wang, Liu and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Pei-Long
Lei, Xiao-Jin
Wang, Yuan-Yuan
Liu, Bai-chao
Wang, Dan-ni
Liu, Zhong-Yuan
Gao, Cai-Qiu
Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title_full Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title_fullStr Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title_full_unstemmed Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title_short Transcriptomic Analysis of Cadmium Stressed Tamarix hispida Revealed Novel Transcripts and the Importance of Abscisic Acid Network
title_sort transcriptomic analysis of cadmium stressed tamarix hispida revealed novel transcripts and the importance of abscisic acid network
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062237/
https://www.ncbi.nlm.nih.gov/pubmed/35519810
http://dx.doi.org/10.3389/fpls.2022.843725
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