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Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine

Several studies have focused on using cell carriers to solve the problem of mesenchymal stem cell expansion on regenerative medicine. However, the disadvantages of using prolonged enzymatic treatment and low cell harvest efficiency still trouble researchers. In this study, PNIPAAm-immobilized gelati...

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Autores principales: Yang, I-Hsuan, Kuan, Che-Yung, Chen, Zhi-Yu, Li, Chi-Han, Chi, Chih-Ying, Lin, Yu-Ying, Liang, Ya-Jyun, Kuo, Wei-Ting, Li, Yi-An, Lin, Feng-Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062325/
https://www.ncbi.nlm.nih.gov/pubmed/35517579
http://dx.doi.org/10.1016/j.mtbio.2022.100266
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author Yang, I-Hsuan
Kuan, Che-Yung
Chen, Zhi-Yu
Li, Chi-Han
Chi, Chih-Ying
Lin, Yu-Ying
Liang, Ya-Jyun
Kuo, Wei-Ting
Li, Yi-An
Lin, Feng-Huei
author_facet Yang, I-Hsuan
Kuan, Che-Yung
Chen, Zhi-Yu
Li, Chi-Han
Chi, Chih-Ying
Lin, Yu-Ying
Liang, Ya-Jyun
Kuo, Wei-Ting
Li, Yi-An
Lin, Feng-Huei
author_sort Yang, I-Hsuan
collection PubMed
description Several studies have focused on using cell carriers to solve the problem of mesenchymal stem cell expansion on regenerative medicine. However, the disadvantages of using prolonged enzymatic treatment and low cell harvest efficiency still trouble researchers. In this study, PNIPAAm-immobilized gelatin microspheres (abbreviated as GNMS) were synthesized using a simple power-driven flow-focusing microinjection system. The developed thermosensitive GNMS can allow easier harvesting of cells from the microspheres, requiring only 10 ​min of low-temperature treatment and 5 ​min of trypsin treatment. The developed GNMS was characterized by Fourier-transform infrared spectroscopy, optical microscopy, and scanning electron microscopy. Further, live/dead staining, F-actin staining, and PrestoBlue cell viability assays were used to evaluate cytotoxicity, cell morphology, cell proliferation, and harvest efficiency. The gene expression of stem cell markers was determined by real-time quantitative PCR (Q-PCR) analysis to investigate the stemness and phenotypic changes in Wharton's jelly-derived mesenchymal stem cells. The results showed that the engineered cell-laden thermosensitive GNMS could significantly increase the cell harvest rate with over 99% cell survival rate and no change in the cell phenotype. Thus, the described strategy GNMS could be the suitable 3D cell carriers in the therapeutic application and opens new avenues for regenerative medicine.
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spelling pubmed-90623252022-05-04 Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine Yang, I-Hsuan Kuan, Che-Yung Chen, Zhi-Yu Li, Chi-Han Chi, Chih-Ying Lin, Yu-Ying Liang, Ya-Jyun Kuo, Wei-Ting Li, Yi-An Lin, Feng-Huei Mater Today Bio Full Length Article Several studies have focused on using cell carriers to solve the problem of mesenchymal stem cell expansion on regenerative medicine. However, the disadvantages of using prolonged enzymatic treatment and low cell harvest efficiency still trouble researchers. In this study, PNIPAAm-immobilized gelatin microspheres (abbreviated as GNMS) were synthesized using a simple power-driven flow-focusing microinjection system. The developed thermosensitive GNMS can allow easier harvesting of cells from the microspheres, requiring only 10 ​min of low-temperature treatment and 5 ​min of trypsin treatment. The developed GNMS was characterized by Fourier-transform infrared spectroscopy, optical microscopy, and scanning electron microscopy. Further, live/dead staining, F-actin staining, and PrestoBlue cell viability assays were used to evaluate cytotoxicity, cell morphology, cell proliferation, and harvest efficiency. The gene expression of stem cell markers was determined by real-time quantitative PCR (Q-PCR) analysis to investigate the stemness and phenotypic changes in Wharton's jelly-derived mesenchymal stem cells. The results showed that the engineered cell-laden thermosensitive GNMS could significantly increase the cell harvest rate with over 99% cell survival rate and no change in the cell phenotype. Thus, the described strategy GNMS could be the suitable 3D cell carriers in the therapeutic application and opens new avenues for regenerative medicine. Elsevier 2022-04-19 /pmc/articles/PMC9062325/ /pubmed/35517579 http://dx.doi.org/10.1016/j.mtbio.2022.100266 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Yang, I-Hsuan
Kuan, Che-Yung
Chen, Zhi-Yu
Li, Chi-Han
Chi, Chih-Ying
Lin, Yu-Ying
Liang, Ya-Jyun
Kuo, Wei-Ting
Li, Yi-An
Lin, Feng-Huei
Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title_full Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title_fullStr Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title_full_unstemmed Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title_short Engineered cell-laden thermosensitive poly(N-isopropylacrylamide)-immobilized gelatin microspheres as 3D cell carriers for regenerative medicine
title_sort engineered cell-laden thermosensitive poly(n-isopropylacrylamide)-immobilized gelatin microspheres as 3d cell carriers for regenerative medicine
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062325/
https://www.ncbi.nlm.nih.gov/pubmed/35517579
http://dx.doi.org/10.1016/j.mtbio.2022.100266
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