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LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b
LncRNAs have been shown to be involved in the biological and pathological processes of acute myeloid leukemia (AML). Hox antisense intergenic RNA myeloid 1 (HOTAIRM1) was reported to be highly expressed in AML. However, the detailed role and molecular mechanism of HOTAIRM1 in AML pathogenesis remain...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062379/ https://www.ncbi.nlm.nih.gov/pubmed/35520918 http://dx.doi.org/10.1039/c9ra00142e |
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author | Hu, Ning Chen, Li Li, Qianyu Zhao, Hongmian |
author_facet | Hu, Ning Chen, Li Li, Qianyu Zhao, Hongmian |
author_sort | Hu, Ning |
collection | PubMed |
description | LncRNAs have been shown to be involved in the biological and pathological processes of acute myeloid leukemia (AML). Hox antisense intergenic RNA myeloid 1 (HOTAIRM1) was reported to be highly expressed in AML. However, the detailed role and molecular mechanism of HOTAIRM1 in AML pathogenesis remain undefined. In the present study, HOTAIRM1 and miR-148b expressions in AML patients and healthy controls were detected by qRT-PCR. Cell proliferation and apoptosis were evaluated by CCK-8 and flow cytometry assays, respectively. The regulatory interaction between HOTAIRM1 and miR-148b was explored by bioinformatics analysis using starBase v3.0 software and The Cancer Genome Atlas (TCGA) AML dataset. We found that the miR-148/miR-152 family members including miR-148a, miR-148b, and miR-152 were predicted to be potential targets of HOTAIRM1. HOTAIRM1 expression was negatively correlated with miR-148b expression but had no correlation with miR-148a/miR-152 expressions in AML samples from the TCGA dataset. HOTAIRM1 expression was higher while miR-148b expression was lower in AML patients than in healthy controls. A negative correlation between HOTAIRM1 and miR-148b in AML patients was observed. HOTAIRM1 silencing and miR-148b overexpression both suppressed cell proliferation and induced apoptosis in AML cells. miR-148b was identified as a target of HOTAIRM1 in AML cells. Moreover, HOTAIRM1 knockdown inhibited proliferation and induced apoptosis in AML cells by negatively regulating miR-148b. In summary, HOTAIRM1 was involved in the progression of AML through targeting miR-148b, shedding light on the biological function and molecular mechanism of HOTAIRM1 in AML. |
format | Online Article Text |
id | pubmed-9062379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90623792022-05-04 LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b Hu, Ning Chen, Li Li, Qianyu Zhao, Hongmian RSC Adv Chemistry LncRNAs have been shown to be involved in the biological and pathological processes of acute myeloid leukemia (AML). Hox antisense intergenic RNA myeloid 1 (HOTAIRM1) was reported to be highly expressed in AML. However, the detailed role and molecular mechanism of HOTAIRM1 in AML pathogenesis remain undefined. In the present study, HOTAIRM1 and miR-148b expressions in AML patients and healthy controls were detected by qRT-PCR. Cell proliferation and apoptosis were evaluated by CCK-8 and flow cytometry assays, respectively. The regulatory interaction between HOTAIRM1 and miR-148b was explored by bioinformatics analysis using starBase v3.0 software and The Cancer Genome Atlas (TCGA) AML dataset. We found that the miR-148/miR-152 family members including miR-148a, miR-148b, and miR-152 were predicted to be potential targets of HOTAIRM1. HOTAIRM1 expression was negatively correlated with miR-148b expression but had no correlation with miR-148a/miR-152 expressions in AML samples from the TCGA dataset. HOTAIRM1 expression was higher while miR-148b expression was lower in AML patients than in healthy controls. A negative correlation between HOTAIRM1 and miR-148b in AML patients was observed. HOTAIRM1 silencing and miR-148b overexpression both suppressed cell proliferation and induced apoptosis in AML cells. miR-148b was identified as a target of HOTAIRM1 in AML cells. Moreover, HOTAIRM1 knockdown inhibited proliferation and induced apoptosis in AML cells by negatively regulating miR-148b. In summary, HOTAIRM1 was involved in the progression of AML through targeting miR-148b, shedding light on the biological function and molecular mechanism of HOTAIRM1 in AML. The Royal Society of Chemistry 2019-04-02 /pmc/articles/PMC9062379/ /pubmed/35520918 http://dx.doi.org/10.1039/c9ra00142e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Hu, Ning Chen, Li Li, Qianyu Zhao, Hongmian LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title | LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title_full | LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title_fullStr | LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title_full_unstemmed | LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title_short | LncRNA HOTAIRM1 is involved in the progression of acute myeloid leukemia through targeting miR-148b |
title_sort | lncrna hotairm1 is involved in the progression of acute myeloid leukemia through targeting mir-148b |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062379/ https://www.ncbi.nlm.nih.gov/pubmed/35520918 http://dx.doi.org/10.1039/c9ra00142e |
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