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A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS

Vitamin D is an important fat-soluble prohormone with pleiotropic effects on human health, such as immunomodulation of the innate and adaptive immune system. There is an unmet clinical need for a rapid screening platform for 25-hydroxyvitamin D (25OH-D) determination without chromatographic separati...

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Autores principales: Helmeczi, Erick, Fries, Eric, Perry, Lauren, Choong, Karen, O’Hearn, Katie, McNally, Dayre, Britz-McKibbin, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062421/
https://www.ncbi.nlm.nih.gov/pubmed/35337847
http://dx.doi.org/10.1016/j.jlr.2022.100204
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author Helmeczi, Erick
Fries, Eric
Perry, Lauren
Choong, Karen
O’Hearn, Katie
McNally, Dayre
Britz-McKibbin, Philip
author_facet Helmeczi, Erick
Fries, Eric
Perry, Lauren
Choong, Karen
O’Hearn, Katie
McNally, Dayre
Britz-McKibbin, Philip
author_sort Helmeczi, Erick
collection PubMed
description Vitamin D is an important fat-soluble prohormone with pleiotropic effects on human health, such as immunomodulation of the innate and adaptive immune system. There is an unmet clinical need for a rapid screening platform for 25-hydroxyvitamin D (25OH-D) determination without chromatographic separation that offers better precision and accuracy than immunoassays. Here, we introduce a high-throughput method for assessing vitamin D status from blood specimens based on direct infusion-MS/MS (DI-MS/MS) following click derivatization using 2-nitrosopyridine. We developed an optimized liquid-phase extraction protocol to minimize ion suppression when directly infusing serum or plasma extracts via a capillary electrophoresis system for quantitative determination of 25OH-D. Acceptable reproducibility (mean coefficient of variation = 10.9%, n = 412), recovery (mean = 102% at 15, 30, and 45 nmol/l), and linearity (R(2) > 0.998) were achieved for 25OH-D with lower detection limits (limit of detection ∼1.2 nmol/l, S/N ∼ 3), greater throughput (∼3 min/sample), and less bias than a commercial chemiluminescence immunoassay prone to batch effects. There was mutual agreement in 25OH-D concentrations from reference blood samples measured by DI-MS/MS as compared with LC-MS/MS (mean bias = 7.8%, n = 18). We also demonstrate that this method could reduce immunoassay misclassification of vitamin D deficiency in a cohort of critically ill children (n = 30). In conclusion, DI-MS/MS offers a viable alternative to LC-MS/MS for assessment of vitamin D status in support of large-scale studies in nutritional epidemiology as well as clinical trials to rapidly screen individual patients who may benefit from vitamin D supplementation.
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spelling pubmed-90624212022-05-03 A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS Helmeczi, Erick Fries, Eric Perry, Lauren Choong, Karen O’Hearn, Katie McNally, Dayre Britz-McKibbin, Philip J Lipid Res Methods Vitamin D is an important fat-soluble prohormone with pleiotropic effects on human health, such as immunomodulation of the innate and adaptive immune system. There is an unmet clinical need for a rapid screening platform for 25-hydroxyvitamin D (25OH-D) determination without chromatographic separation that offers better precision and accuracy than immunoassays. Here, we introduce a high-throughput method for assessing vitamin D status from blood specimens based on direct infusion-MS/MS (DI-MS/MS) following click derivatization using 2-nitrosopyridine. We developed an optimized liquid-phase extraction protocol to minimize ion suppression when directly infusing serum or plasma extracts via a capillary electrophoresis system for quantitative determination of 25OH-D. Acceptable reproducibility (mean coefficient of variation = 10.9%, n = 412), recovery (mean = 102% at 15, 30, and 45 nmol/l), and linearity (R(2) > 0.998) were achieved for 25OH-D with lower detection limits (limit of detection ∼1.2 nmol/l, S/N ∼ 3), greater throughput (∼3 min/sample), and less bias than a commercial chemiluminescence immunoassay prone to batch effects. There was mutual agreement in 25OH-D concentrations from reference blood samples measured by DI-MS/MS as compared with LC-MS/MS (mean bias = 7.8%, n = 18). We also demonstrate that this method could reduce immunoassay misclassification of vitamin D deficiency in a cohort of critically ill children (n = 30). In conclusion, DI-MS/MS offers a viable alternative to LC-MS/MS for assessment of vitamin D status in support of large-scale studies in nutritional epidemiology as well as clinical trials to rapidly screen individual patients who may benefit from vitamin D supplementation. American Society for Biochemistry and Molecular Biology 2022-03-23 /pmc/articles/PMC9062421/ /pubmed/35337847 http://dx.doi.org/10.1016/j.jlr.2022.100204 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Methods
Helmeczi, Erick
Fries, Eric
Perry, Lauren
Choong, Karen
O’Hearn, Katie
McNally, Dayre
Britz-McKibbin, Philip
A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title_full A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title_fullStr A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title_full_unstemmed A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title_short A high-throughput platform for the rapid screening of vitamin D status by direct infusion-MS/MS
title_sort high-throughput platform for the rapid screening of vitamin d status by direct infusion-ms/ms
topic Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062421/
https://www.ncbi.nlm.nih.gov/pubmed/35337847
http://dx.doi.org/10.1016/j.jlr.2022.100204
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