The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We...

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Detalles Bibliográficos
Autores principales: Wan, Jieyu, Li, Fangjie, Wang, Mingshu, Cheng, Anchun, Tian, Bin, Yang, Qiao, Wu, Ying, Ou, Xumin, Mao, Sai, Sun, Di, Zhang, Shaqiu, Huang, Juan, Gao, Qun, Zhao, XinXin, Chen, Shun, Liu, Mafeng, Jia, Renyong, Zhu, Dekang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
IMMUNOLOGY, HEALTH AND DISEASE
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062478/
https://www.ncbi.nlm.nih.gov/pubmed/35489250
http://dx.doi.org/10.1016/j.psj.2022.101863
Descripción
Sumario:To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.

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