The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We...

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Detalles Bibliográficos
Autores principales: Wan, Jieyu, Li, Fangjie, Wang, Mingshu, Cheng, Anchun, Tian, Bin, Yang, Qiao, Wu, Ying, Ou, Xumin, Mao, Sai, Sun, Di, Zhang, Shaqiu, Huang, Juan, Gao, Qun, Zhao, XinXin, Chen, Shun, Liu, Mafeng, Jia, Renyong, Zhu, Dekang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
IMMUNOLOGY, HEALTH AND DISEASE
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062478/
https://www.ncbi.nlm.nih.gov/pubmed/35489250
http://dx.doi.org/10.1016/j.psj.2022.101863
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author Wan, Jieyu
Li, Fangjie
Wang, Mingshu
Cheng, Anchun
Tian, Bin
Yang, Qiao
Wu, Ying
Ou, Xumin
Mao, Sai
Sun, Di
Zhang, Shaqiu
Huang, Juan
Gao, Qun
Zhao, XinXin
Chen, Shun
Liu, Mafeng
Jia, Renyong
Zhu, Dekang
author_facet Wan, Jieyu
Li, Fangjie
Wang, Mingshu
Cheng, Anchun
Tian, Bin
Yang, Qiao
Wu, Ying
Ou, Xumin
Mao, Sai
Sun, Di
Zhang, Shaqiu
Huang, Juan
Gao, Qun
Zhao, XinXin
Chen, Shun
Liu, Mafeng
Jia, Renyong
Zhu, Dekang
author_sort Wan, Jieyu
collection PubMed
description To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.
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spelling pubmed-90624782022-05-04 The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication Wan, Jieyu Li, Fangjie Wang, Mingshu Cheng, Anchun Tian, Bin Yang, Qiao Wu, Ying Ou, Xumin Mao, Sai Sun, Di Zhang, Shaqiu Huang, Juan Gao, Qun Zhao, XinXin Chen, Shun Liu, Mafeng Jia, Renyong Zhu, Dekang Poult Sci IMMUNOLOGY, HEALTH AND DISEASE To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication. Elsevier 2022-03-17 /pmc/articles/PMC9062478/ /pubmed/35489250 http://dx.doi.org/10.1016/j.psj.2022.101863 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Wan, Jieyu
Li, Fangjie
Wang, Mingshu
Cheng, Anchun
Tian, Bin
Yang, Qiao
Wu, Ying
Ou, Xumin
Mao, Sai
Sun, Di
Zhang, Shaqiu
Huang, Juan
Gao, Qun
Zhao, XinXin
Chen, Shun
Liu, Mafeng
Jia, Renyong
Zhu, Dekang
The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title_full The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title_fullStr The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title_full_unstemmed The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title_short The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication
title_sort protein encoded by the duck plague virus ul14 gene regulates virion morphogenesis and affects viral replication
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062478/
https://www.ncbi.nlm.nih.gov/pubmed/35489250
http://dx.doi.org/10.1016/j.psj.2022.101863
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