Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression

Enterotoxins (SEs) produced by Staphylococcus aureus are the cause of serious food intoxications. Staphylococcal enterotoxin C (SEC) is one of the main contributors, as it is often highly expressed. S. aureus possesses a competitive growth advantage over accompanying bacterial flora under stress con...

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Autores principales: Etter, Danai, Ukowitz, Christina, Eicher, Corinne, Tasara, Taurai, Johler, Sophia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062481/
https://www.ncbi.nlm.nih.gov/pubmed/35516428
http://dx.doi.org/10.3389/fmicb.2022.820067
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author Etter, Danai
Ukowitz, Christina
Eicher, Corinne
Tasara, Taurai
Johler, Sophia
author_facet Etter, Danai
Ukowitz, Christina
Eicher, Corinne
Tasara, Taurai
Johler, Sophia
author_sort Etter, Danai
collection PubMed
description Enterotoxins (SEs) produced by Staphylococcus aureus are the cause of serious food intoxications. Staphylococcal enterotoxin C (SEC) is one of the main contributors, as it is often highly expressed. S. aureus possesses a competitive growth advantage over accompanying bacterial flora under stress conditions encountered in foods, such as high NaCl concentrations. However, the influence of NaCl as an external stressor on SEC expression is still unclear. We investigated the influence of 4.5% NaCl on sec mRNA and SEC protein levels. A qRT-PCR assay revealed that NaCl stress leads to time-dependently decreased or elevated sec mRNA levels for most strains. SEC protein levels were generally decreased under NaCl stress. Our findings suggest that NaCl stress lowers overall SEC concentration and time-dependently affects sec mRNA levels.
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spelling pubmed-90624812022-05-04 Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression Etter, Danai Ukowitz, Christina Eicher, Corinne Tasara, Taurai Johler, Sophia Front Microbiol Microbiology Enterotoxins (SEs) produced by Staphylococcus aureus are the cause of serious food intoxications. Staphylococcal enterotoxin C (SEC) is one of the main contributors, as it is often highly expressed. S. aureus possesses a competitive growth advantage over accompanying bacterial flora under stress conditions encountered in foods, such as high NaCl concentrations. However, the influence of NaCl as an external stressor on SEC expression is still unclear. We investigated the influence of 4.5% NaCl on sec mRNA and SEC protein levels. A qRT-PCR assay revealed that NaCl stress leads to time-dependently decreased or elevated sec mRNA levels for most strains. SEC protein levels were generally decreased under NaCl stress. Our findings suggest that NaCl stress lowers overall SEC concentration and time-dependently affects sec mRNA levels. Frontiers Media S.A. 2022-04-18 /pmc/articles/PMC9062481/ /pubmed/35516428 http://dx.doi.org/10.3389/fmicb.2022.820067 Text en Copyright © 2022 Etter, Ukowitz, Eicher, Tasara and Johler. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Etter, Danai
Ukowitz, Christina
Eicher, Corinne
Tasara, Taurai
Johler, Sophia
Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title_full Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title_fullStr Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title_full_unstemmed Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title_short Mild NaCl Stress Influences Staphylococcal Enterotoxin C Transcription in a Time-Dependent Manner and Reduces Protein Expression
title_sort mild nacl stress influences staphylococcal enterotoxin c transcription in a time-dependent manner and reduces protein expression
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062481/
https://www.ncbi.nlm.nih.gov/pubmed/35516428
http://dx.doi.org/10.3389/fmicb.2022.820067
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