Cargando…

Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD

Avian pathogenic Escherichia coli (APEC) is an important pathogen causing several diseases in birds. It is responsible for local and systemic infections in poultry, seriously impeding the development of the poultry industry, and poses a potential risk to public health. The iron absorption regulatory...

Descripción completa

Detalles Bibliográficos
Autores principales: Niu, Lulu, Cai, Weizhen, Cheng, Xi, Li, Zhe, Ruan, Jianming, Li, Fangguo, Qi, Kezong, Tu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062578/
https://www.ncbi.nlm.nih.gov/pubmed/35518642
http://dx.doi.org/10.3389/fvets.2022.854916
_version_ 1784698975463407616
author Niu, Lulu
Cai, Weizhen
Cheng, Xi
Li, Zhe
Ruan, Jianming
Li, Fangguo
Qi, Kezong
Tu, Jian
author_facet Niu, Lulu
Cai, Weizhen
Cheng, Xi
Li, Zhe
Ruan, Jianming
Li, Fangguo
Qi, Kezong
Tu, Jian
author_sort Niu, Lulu
collection PubMed
description Avian pathogenic Escherichia coli (APEC) is an important pathogen causing several diseases in birds. It is responsible for local and systemic infections in poultry, seriously impeding the development of the poultry industry, and poses a potential risk to public health. The iron absorption regulatory protein Fur and the noncoding RNA, RyhB, that it negatively regulates are important factors in bacterial iron uptake, but the regulation of bacterial virulence genes varies greatly among different bacteria. We found that Fur is very important for the mobility of APEC. The expression of fur and RyhB is extensively regulated in APEC, and RyhB expression is also negatively regulated by Fur. A transcriptomic analysis showed that the genes significantly differentially regulated by Fur are related to cell movement, including pilus- or flagellum-dependent cell motility. To verify these results, we examined the effects of fur knockdown on cell movement by measuring the diameter of the bacteria colonies. Consistent with the RNA sequencing results, the mobility of AE17Δfur was significantly reduced compared with that of the wild type, and it had almost lost its ability to move. Using an electrophoretic mobility assay, we confirmed that the Fur protein directly binds to the promoter region of the key flagellum-related gene flhD, thereby affecting the assembly and synthesis of the APEC flagellum. This study extends our understanding of gene regulation in APEC.
format Online
Article
Text
id pubmed-9062578
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90625782022-05-04 Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD Niu, Lulu Cai, Weizhen Cheng, Xi Li, Zhe Ruan, Jianming Li, Fangguo Qi, Kezong Tu, Jian Front Vet Sci Veterinary Science Avian pathogenic Escherichia coli (APEC) is an important pathogen causing several diseases in birds. It is responsible for local and systemic infections in poultry, seriously impeding the development of the poultry industry, and poses a potential risk to public health. The iron absorption regulatory protein Fur and the noncoding RNA, RyhB, that it negatively regulates are important factors in bacterial iron uptake, but the regulation of bacterial virulence genes varies greatly among different bacteria. We found that Fur is very important for the mobility of APEC. The expression of fur and RyhB is extensively regulated in APEC, and RyhB expression is also negatively regulated by Fur. A transcriptomic analysis showed that the genes significantly differentially regulated by Fur are related to cell movement, including pilus- or flagellum-dependent cell motility. To verify these results, we examined the effects of fur knockdown on cell movement by measuring the diameter of the bacteria colonies. Consistent with the RNA sequencing results, the mobility of AE17Δfur was significantly reduced compared with that of the wild type, and it had almost lost its ability to move. Using an electrophoretic mobility assay, we confirmed that the Fur protein directly binds to the promoter region of the key flagellum-related gene flhD, thereby affecting the assembly and synthesis of the APEC flagellum. This study extends our understanding of gene regulation in APEC. Frontiers Media S.A. 2022-04-18 /pmc/articles/PMC9062578/ /pubmed/35518642 http://dx.doi.org/10.3389/fvets.2022.854916 Text en Copyright © 2022 Niu, Cai, Cheng, Li, Ruan, Li, Qi and Tu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Niu, Lulu
Cai, Weizhen
Cheng, Xi
Li, Zhe
Ruan, Jianming
Li, Fangguo
Qi, Kezong
Tu, Jian
Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title_full Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title_fullStr Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title_full_unstemmed Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title_short Fur Protein Regulates the Motility of Avian Pathogenic Escherichia coli AE17 Through Promoter Regions of the Flagella Key Genes flhD
title_sort fur protein regulates the motility of avian pathogenic escherichia coli ae17 through promoter regions of the flagella key genes flhd
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062578/
https://www.ncbi.nlm.nih.gov/pubmed/35518642
http://dx.doi.org/10.3389/fvets.2022.854916
work_keys_str_mv AT niululu furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT caiweizhen furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT chengxi furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT lizhe furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT ruanjianming furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT lifangguo furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT qikezong furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd
AT tujian furproteinregulatesthemotilityofavianpathogenicescherichiacoliae17throughpromoterregionsoftheflagellakeygenesflhd