Open source fraction collector/MALDI spotter for proteomics

We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the...

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Detalles Bibliográficos
Autores principales: Ficarro, Scott B., Max Alexander, William, Tavares, Isidoro, Marto, Jarrod A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062586/
https://www.ncbi.nlm.nih.gov/pubmed/35518277
http://dx.doi.org/10.1016/j.ohx.2022.e00305
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author Ficarro, Scott B.
Max Alexander, William
Tavares, Isidoro
Marto, Jarrod A.
author_facet Ficarro, Scott B.
Max Alexander, William
Tavares, Isidoro
Marto, Jarrod A.
author_sort Ficarro, Scott B.
collection PubMed
description We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the cost compared to typical commercial systems. Control software is written in Python allowing for rapid customization. We demonstrate several useful applications, including the automated deposition of LC separated peptides for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) as well as collection and concatenation of peptide fractions from nanoflow HPLC separations.
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spelling pubmed-90625862022-05-04 Open source fraction collector/MALDI spotter for proteomics Ficarro, Scott B. Max Alexander, William Tavares, Isidoro Marto, Jarrod A. HardwareX Article We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the cost compared to typical commercial systems. Control software is written in Python allowing for rapid customization. We demonstrate several useful applications, including the automated deposition of LC separated peptides for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) as well as collection and concatenation of peptide fractions from nanoflow HPLC separations. Elsevier 2022-04-18 /pmc/articles/PMC9062586/ /pubmed/35518277 http://dx.doi.org/10.1016/j.ohx.2022.e00305 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ficarro, Scott B.
Max Alexander, William
Tavares, Isidoro
Marto, Jarrod A.
Open source fraction collector/MALDI spotter for proteomics
title Open source fraction collector/MALDI spotter for proteomics
title_full Open source fraction collector/MALDI spotter for proteomics
title_fullStr Open source fraction collector/MALDI spotter for proteomics
title_full_unstemmed Open source fraction collector/MALDI spotter for proteomics
title_short Open source fraction collector/MALDI spotter for proteomics
title_sort open source fraction collector/maldi spotter for proteomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062586/
https://www.ncbi.nlm.nih.gov/pubmed/35518277
http://dx.doi.org/10.1016/j.ohx.2022.e00305
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