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Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)

Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed....

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Autores principales: Wang, Dangfeng, Cui, Fangchao, Ren, Likun, Tan, Xiqian, Lv, Xinran, Li, Qiuying, Li, Jianrong, Li, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062736/
https://www.ncbi.nlm.nih.gov/pubmed/35516425
http://dx.doi.org/10.3389/fmicb.2022.856802
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author Wang, Dangfeng
Cui, Fangchao
Ren, Likun
Tan, Xiqian
Lv, Xinran
Li, Qiuying
Li, Jianrong
Li, Tingting
author_facet Wang, Dangfeng
Cui, Fangchao
Ren, Likun
Tan, Xiqian
Lv, Xinran
Li, Qiuying
Li, Jianrong
Li, Tingting
author_sort Wang, Dangfeng
collection PubMed
description Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of P. fluorescens PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of P. fluorescens PF08. Results of quantitative real-time PCR revealed the key role of the P. fluorescens PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of P. fluorescens PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference.
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spelling pubmed-90627362022-05-04 Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus) Wang, Dangfeng Cui, Fangchao Ren, Likun Tan, Xiqian Lv, Xinran Li, Qiuying Li, Jianrong Li, Tingting Front Microbiol Microbiology Pseudomonas fluorescens is a common specific spoilage organism (SSO) of aquatic products. The spoilage ability of SSO can be regulated by the quorum sensing (QS) system. However, the QS system in P. fluorescens and their relationship with the spoilage potential have not been systematically analyzed. In the present study, the complete genome of P. fluorescens PF08 isolated from spoilage turbot was sequenced. The identification of key genes that involved in the QS, enzyme synthesis, sulfur, and amino acid metabolism explained the spoilage potential of P. fluorescens PF08. Results of quantitative real-time PCR revealed the key role of the P. fluorescens PF08 QS system in regulating the transcription of spoilage-related genes and its sensitivity to environmental stress. These findings provide insight into the spoilage features of P. fluorescens PF08 from a genomic perspective. The knowledge may be valuable in the development of new strategies for the targeted inhibition of aquatic product spoilage based on QS interference. Frontiers Media S.A. 2022-04-18 /pmc/articles/PMC9062736/ /pubmed/35516425 http://dx.doi.org/10.3389/fmicb.2022.856802 Text en Copyright © 2022 Wang, Cui, Ren, Tan, Lv, Li, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Dangfeng
Cui, Fangchao
Ren, Likun
Tan, Xiqian
Lv, Xinran
Li, Qiuying
Li, Jianrong
Li, Tingting
Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title_full Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title_fullStr Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title_full_unstemmed Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title_short Complete Genome Analysis Reveals the Quorum Sensing-Related Spoilage Potential of Pseudomonas fluorescens PF08, a Specific Spoilage Organism of Turbot (Scophthalmus maximus)
title_sort complete genome analysis reveals the quorum sensing-related spoilage potential of pseudomonas fluorescens pf08, a specific spoilage organism of turbot (scophthalmus maximus)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062736/
https://www.ncbi.nlm.nih.gov/pubmed/35516425
http://dx.doi.org/10.3389/fmicb.2022.856802
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