Enhanced Detection of Viral RNA Species Using FokI-Assisted Digestion of DNA Duplexes and DNA/RNA Hybrids

[Image: see text] The accurate detection of nucleic acids from certain biological pathogens is critical for the diagnosis of human diseases. However, amplified detection of RNA molecules from a complex sample by direct detection of RNA/DNA hybrids remains a challenge. Here, we show that type IIS end...

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Detalles Bibliográficos
Autores principales: Tejedor, Juan R., Martín, Gabriel, Roberti, Annalisa, Mangas, Cristina, Santamarina-Ojeda, Pablo, Fernández Pérez, Raúl, López, Virginia, González Urdinguio, Rocío, Alba-Linares, Juan J., Peñarroya, Alfonso, Álvarez-Argüelles, Marta E., Boga, José A., Fernández Fernández, Agustín, Rojo-Alba, Susana, Fernández Fraga, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063116/
https://www.ncbi.nlm.nih.gov/pubmed/35467835
http://dx.doi.org/10.1021/acs.analchem.2c00407

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