Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites
BACKGROUND: Oil spills are ranked among the greatest global challenges to humanity. In Uganda, owing to the forthcoming full-scale production of multi-billion barrels of oil, the country’s oil pollution burden is anticipated to escalate, necessitating remediation. Due to the unsuitability of several...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063239/ https://www.ncbi.nlm.nih.gov/pubmed/35505298 http://dx.doi.org/10.1186/s12866-022-02541-x |
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author | Ssenku, Jamilu E. Walusansa, Abdul Oryem-Origa, Hannington Ssemanda, Paul Ntambi, Saidi Omujal, Francis Mustafa, Abubakar Sadik |
author_facet | Ssenku, Jamilu E. Walusansa, Abdul Oryem-Origa, Hannington Ssemanda, Paul Ntambi, Saidi Omujal, Francis Mustafa, Abubakar Sadik |
author_sort | Ssenku, Jamilu E. |
collection | PubMed |
description | BACKGROUND: Oil spills are ranked among the greatest global challenges to humanity. In Uganda, owing to the forthcoming full-scale production of multi-billion barrels of oil, the country’s oil pollution burden is anticipated to escalate, necessitating remediation. Due to the unsuitability of several oil clean-up technologies, the search for cost-effective and environmentally friendly remediation technologies is paramount. We thus carried out this study to examine the occurrence of metabolically active indigenous bacterial species and chemical characteristics of soils with a long history of oil pollution in Uganda that can be used in the development of a bacterial-based product for remediation of oil-polluted sites. RESULTS: Total hydrocarbon analysis of the soil samples revealed that the three most abundant hydrocarbons were pyrene, anthracene and phenanthrene that were significantly higher in oil-polluted sites than in the control sites. Using the BIOLOG EcoPlate™, the study revealed that bacterial species richness, bacterial diversity and bacterial activity (ANOVA, p < 0.05) significantly varied among the sites. Only bacterial activity showed significant variation across the three cities (ANOVA, p < 0.05). Additionally, the study revealed significant moderate positive correlation between the bacterial community profiles with Zn and organic contents while correlations between the bacterial community profiles and the hydrocarbons were largely moderate and positively correlated. CONCLUSIONS: This study revealed largely similar bacterial community profiles between the oil-polluted and control sites suggestive of the occurrence of metabolically active bacterial populations in both sites. The oil-polluted sites had higher petroleum hydrocarbon, heavy metal, nitrogen and phosphorus contents. Even though we observed similar bacterial community profiles between the oil polluted and control sites, the actual bacterial community composition may be different, owing to a higher exposure to petroleum hydrocarbons. However, the existence of oil degrading bacteria in unpolluted soils should not be overlooked. Thus, there is a need to ascertain the actual indigenous bacterial populations with potential to degrade hydrocarbons from both oil-polluted and unpolluted sites in Uganda to inform the design and development of a bacterial-based oil remediation product that could be used to manage the imminent pollution from oil exploration and increased utilization of petroleum products in Uganda. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02541-x. |
format | Online Article Text |
id | pubmed-9063239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90632392022-05-04 Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites Ssenku, Jamilu E. Walusansa, Abdul Oryem-Origa, Hannington Ssemanda, Paul Ntambi, Saidi Omujal, Francis Mustafa, Abubakar Sadik BMC Microbiol Research BACKGROUND: Oil spills are ranked among the greatest global challenges to humanity. In Uganda, owing to the forthcoming full-scale production of multi-billion barrels of oil, the country’s oil pollution burden is anticipated to escalate, necessitating remediation. Due to the unsuitability of several oil clean-up technologies, the search for cost-effective and environmentally friendly remediation technologies is paramount. We thus carried out this study to examine the occurrence of metabolically active indigenous bacterial species and chemical characteristics of soils with a long history of oil pollution in Uganda that can be used in the development of a bacterial-based product for remediation of oil-polluted sites. RESULTS: Total hydrocarbon analysis of the soil samples revealed that the three most abundant hydrocarbons were pyrene, anthracene and phenanthrene that were significantly higher in oil-polluted sites than in the control sites. Using the BIOLOG EcoPlate™, the study revealed that bacterial species richness, bacterial diversity and bacterial activity (ANOVA, p < 0.05) significantly varied among the sites. Only bacterial activity showed significant variation across the three cities (ANOVA, p < 0.05). Additionally, the study revealed significant moderate positive correlation between the bacterial community profiles with Zn and organic contents while correlations between the bacterial community profiles and the hydrocarbons were largely moderate and positively correlated. CONCLUSIONS: This study revealed largely similar bacterial community profiles between the oil-polluted and control sites suggestive of the occurrence of metabolically active bacterial populations in both sites. The oil-polluted sites had higher petroleum hydrocarbon, heavy metal, nitrogen and phosphorus contents. Even though we observed similar bacterial community profiles between the oil polluted and control sites, the actual bacterial community composition may be different, owing to a higher exposure to petroleum hydrocarbons. However, the existence of oil degrading bacteria in unpolluted soils should not be overlooked. Thus, there is a need to ascertain the actual indigenous bacterial populations with potential to degrade hydrocarbons from both oil-polluted and unpolluted sites in Uganda to inform the design and development of a bacterial-based oil remediation product that could be used to manage the imminent pollution from oil exploration and increased utilization of petroleum products in Uganda. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02541-x. BioMed Central 2022-05-03 /pmc/articles/PMC9063239/ /pubmed/35505298 http://dx.doi.org/10.1186/s12866-022-02541-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ssenku, Jamilu E. Walusansa, Abdul Oryem-Origa, Hannington Ssemanda, Paul Ntambi, Saidi Omujal, Francis Mustafa, Abubakar Sadik Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title | Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title_full | Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title_fullStr | Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title_full_unstemmed | Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title_short | Bacterial community and chemical profiles of oil-polluted sites in selected cities of Uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
title_sort | bacterial community and chemical profiles of oil-polluted sites in selected cities of uganda: potential for developing a bacterial-based product for remediation of oil-polluted sites |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063239/ https://www.ncbi.nlm.nih.gov/pubmed/35505298 http://dx.doi.org/10.1186/s12866-022-02541-x |
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