Single-molecule study on conformational dynamics of M.HhaI

We found that apo DNA methyltransferase M.HhaI under the physiological salt concentration does not possess the structure characterized by X-ray crystallography; instead, it interchanges between prefolded and unfolded states. Only after binding to the substrate, it transforms into a crystal-structure...

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Detalles Bibliográficos
Autores principales: He, Shanshan, Yang, Chen, Peng, Sijia, Chen, Chunlai, Zhao, Xin Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064139/
https://www.ncbi.nlm.nih.gov/pubmed/35516350
http://dx.doi.org/10.1039/c9ra00021f
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author He, Shanshan
Yang, Chen
Peng, Sijia
Chen, Chunlai
Zhao, Xin Sheng
author_facet He, Shanshan
Yang, Chen
Peng, Sijia
Chen, Chunlai
Zhao, Xin Sheng
author_sort He, Shanshan
collection PubMed
description We found that apo DNA methyltransferase M.HhaI under the physiological salt concentration does not possess the structure characterized by X-ray crystallography; instead, it interchanges between prefolded and unfolded states. Only after binding to the substrate, it transforms into a crystal-structure-like state. Flipping rates of its catalytic loop were directly measured.
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spelling pubmed-90641392022-05-04 Single-molecule study on conformational dynamics of M.HhaI He, Shanshan Yang, Chen Peng, Sijia Chen, Chunlai Zhao, Xin Sheng RSC Adv Chemistry We found that apo DNA methyltransferase M.HhaI under the physiological salt concentration does not possess the structure characterized by X-ray crystallography; instead, it interchanges between prefolded and unfolded states. Only after binding to the substrate, it transforms into a crystal-structure-like state. Flipping rates of its catalytic loop were directly measured. The Royal Society of Chemistry 2019-05-13 /pmc/articles/PMC9064139/ /pubmed/35516350 http://dx.doi.org/10.1039/c9ra00021f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
He, Shanshan
Yang, Chen
Peng, Sijia
Chen, Chunlai
Zhao, Xin Sheng
Single-molecule study on conformational dynamics of M.HhaI
title Single-molecule study on conformational dynamics of M.HhaI
title_full Single-molecule study on conformational dynamics of M.HhaI
title_fullStr Single-molecule study on conformational dynamics of M.HhaI
title_full_unstemmed Single-molecule study on conformational dynamics of M.HhaI
title_short Single-molecule study on conformational dynamics of M.HhaI
title_sort single-molecule study on conformational dynamics of m.hhai
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064139/
https://www.ncbi.nlm.nih.gov/pubmed/35516350
http://dx.doi.org/10.1039/c9ra00021f
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