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A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications
Development of high performance fluorescent chemosensors for the detection of ClO(−)in vitro and in vivo is very desirable, because many human diseases are caused by ClO(−). In this paper, a highly selectivity and sensitive fluorescent probe, EDPC, based on 3-acetylcoumarin, was synthesized, which c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064325/ https://www.ncbi.nlm.nih.gov/pubmed/35521388 http://dx.doi.org/10.1039/c9ra01457h |
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author | Jin, Lei Tan, Xiaoxue Dai, Lihui Sheng, Liqiang Wang, Qingming |
author_facet | Jin, Lei Tan, Xiaoxue Dai, Lihui Sheng, Liqiang Wang, Qingming |
author_sort | Jin, Lei |
collection | PubMed |
description | Development of high performance fluorescent chemosensors for the detection of ClO(−)in vitro and in vivo is very desirable, because many human diseases are caused by ClO(−). In this paper, a highly selectivity and sensitive fluorescent probe, EDPC, based on 3-acetylcoumarin, was synthesized, which could respond to ClO(−) and exhibit an “off–on” mode in Tris–HCl buffer (pH = 7.2, 10 mM, 50% C(2)H(5)OH) solutions. The detection limit of the EDPC probe for ClO(−) was as low as 1.2 × 10(−8) M. Moreover, the high selectivity and high sensitivity of EDPC towards ClO(−) are attributed to the oxidation reaction between the C–O of the coumarin lactone and the C[double bond, length as m-dash]C formed by aldol condensation and the mechanism was further verified using ESI-MS and DFT. Additionally, the concentrations of ClO(−) in real water were also calculated using the EDPC probe and showed good recovery. Finally, the distribution of intracellular endogenous ClO(−) was gained by confocal fluorescence microscopy in living HEK293T cells. |
format | Online Article Text |
id | pubmed-9064325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90643252022-05-04 A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications Jin, Lei Tan, Xiaoxue Dai, Lihui Sheng, Liqiang Wang, Qingming RSC Adv Chemistry Development of high performance fluorescent chemosensors for the detection of ClO(−)in vitro and in vivo is very desirable, because many human diseases are caused by ClO(−). In this paper, a highly selectivity and sensitive fluorescent probe, EDPC, based on 3-acetylcoumarin, was synthesized, which could respond to ClO(−) and exhibit an “off–on” mode in Tris–HCl buffer (pH = 7.2, 10 mM, 50% C(2)H(5)OH) solutions. The detection limit of the EDPC probe for ClO(−) was as low as 1.2 × 10(−8) M. Moreover, the high selectivity and high sensitivity of EDPC towards ClO(−) are attributed to the oxidation reaction between the C–O of the coumarin lactone and the C[double bond, length as m-dash]C formed by aldol condensation and the mechanism was further verified using ESI-MS and DFT. Additionally, the concentrations of ClO(−) in real water were also calculated using the EDPC probe and showed good recovery. Finally, the distribution of intracellular endogenous ClO(−) was gained by confocal fluorescence microscopy in living HEK293T cells. The Royal Society of Chemistry 2019-05-21 /pmc/articles/PMC9064325/ /pubmed/35521388 http://dx.doi.org/10.1039/c9ra01457h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Jin, Lei Tan, Xiaoxue Dai, Lihui Sheng, Liqiang Wang, Qingming A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title | A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title_full | A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title_fullStr | A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title_full_unstemmed | A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title_short | A highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
title_sort | highly specific and sensitive turn-on fluorescence probe for hypochlorite detection and its bioimaging applications |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064325/ https://www.ncbi.nlm.nih.gov/pubmed/35521388 http://dx.doi.org/10.1039/c9ra01457h |
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