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Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane

Porcine decellularized small intestine submucosa (SIS) is a collagen membrane, which offers great potential as an organic substrate template in mineralization processes due to its good biodegradability and biocompatibility. However, a long period of mineralization and low efficiency are apparent, an...

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Autores principales: Zhu, Qiuhong, Jiao, Hua, Zhao, Xiaoliang, Tang, Yufei, Zhao, Kang, Gou, Xingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064378/
https://www.ncbi.nlm.nih.gov/pubmed/35520114
http://dx.doi.org/10.1039/d2ra00910b
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author Zhu, Qiuhong
Jiao, Hua
Zhao, Xiaoliang
Tang, Yufei
Zhao, Kang
Gou, Xingchun
author_facet Zhu, Qiuhong
Jiao, Hua
Zhao, Xiaoliang
Tang, Yufei
Zhao, Kang
Gou, Xingchun
author_sort Zhu, Qiuhong
collection PubMed
description Porcine decellularized small intestine submucosa (SIS) is a collagen membrane, which offers great potential as an organic substrate template in mineralization processes due to its good biodegradability and biocompatibility. However, a long period of mineralization and low efficiency are apparent, and the mechanism of collagen fiber mineralization has often been neglected in the previous literature. Thus, in this paper, we present a novel model of biomimetic collagen mineralization which uses dopamine (DA) molecules with the activating and retouching function of SIS collagen membranes and regulating collagen mineralization to construct the structure of mineralized collagen hard tissues. The crystal biomimetic mineralization growth of calcium phosphate on membranes is studied in different solid–liquid interfaces with a double ion self-assembled diffusion system under the simulated physiological microenvironment. In the system, pDA@SIS membranes are used to control the concentration of Ca(2+) and PO(4)(3−) ionic diffusion to generate supersaturation reaction conditions in 1–14 days. The system can successfully obtain polycrystals with low crystallinity on the pDA-collagen complex template surface of collagen fibers and along the collagen fibers. It initiates a generalized bionic mineralization pathway which can reduce the nucleation interfacial energy to promote rapid hydroxyapatite (HAP) nucleation and crystallization and accelerate the rate of collagen fiber mineralization. The pDA@SIS mineralized collagen membrane shows good biocompatibility with 100% cellular activity in the CCK-8 test, which significantly improved the adhesion proliferation of MC3T3-E1 cells. The pDA-SIS collagen complex, as a new type of mineralization template, may propose a new collagen mineralization strategy to produce a mineralized pDA@SIS scaffold bone-like material for tissue engineering or can potentially be applied in bone repair and regeneration.
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spelling pubmed-90643782022-05-04 Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane Zhu, Qiuhong Jiao, Hua Zhao, Xiaoliang Tang, Yufei Zhao, Kang Gou, Xingchun RSC Adv Chemistry Porcine decellularized small intestine submucosa (SIS) is a collagen membrane, which offers great potential as an organic substrate template in mineralization processes due to its good biodegradability and biocompatibility. However, a long period of mineralization and low efficiency are apparent, and the mechanism of collagen fiber mineralization has often been neglected in the previous literature. Thus, in this paper, we present a novel model of biomimetic collagen mineralization which uses dopamine (DA) molecules with the activating and retouching function of SIS collagen membranes and regulating collagen mineralization to construct the structure of mineralized collagen hard tissues. The crystal biomimetic mineralization growth of calcium phosphate on membranes is studied in different solid–liquid interfaces with a double ion self-assembled diffusion system under the simulated physiological microenvironment. In the system, pDA@SIS membranes are used to control the concentration of Ca(2+) and PO(4)(3−) ionic diffusion to generate supersaturation reaction conditions in 1–14 days. The system can successfully obtain polycrystals with low crystallinity on the pDA-collagen complex template surface of collagen fibers and along the collagen fibers. It initiates a generalized bionic mineralization pathway which can reduce the nucleation interfacial energy to promote rapid hydroxyapatite (HAP) nucleation and crystallization and accelerate the rate of collagen fiber mineralization. The pDA@SIS mineralized collagen membrane shows good biocompatibility with 100% cellular activity in the CCK-8 test, which significantly improved the adhesion proliferation of MC3T3-E1 cells. The pDA-SIS collagen complex, as a new type of mineralization template, may propose a new collagen mineralization strategy to produce a mineralized pDA@SIS scaffold bone-like material for tissue engineering or can potentially be applied in bone repair and regeneration. The Royal Society of Chemistry 2022-05-03 /pmc/articles/PMC9064378/ /pubmed/35520114 http://dx.doi.org/10.1039/d2ra00910b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhu, Qiuhong
Jiao, Hua
Zhao, Xiaoliang
Tang, Yufei
Zhao, Kang
Gou, Xingchun
Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title_full Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title_fullStr Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title_full_unstemmed Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title_short Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane
title_sort hydroxyapatite formation in biomimetic synthesis with the interface of a pda@sis membrane
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064378/
https://www.ncbi.nlm.nih.gov/pubmed/35520114
http://dx.doi.org/10.1039/d2ra00910b
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