Cargando…

Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship

To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zejun, Chen, Lijuan, Cui, Xiuming, Zhang, Yiming, Hu, Yupiao, Wang, Chengxiao, Xiong, Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064609/
https://www.ncbi.nlm.nih.gov/pubmed/35520546
http://dx.doi.org/10.1039/c9ra00906j
_version_ 1784699417932070912
author Zhang, Zejun
Chen, Lijuan
Cui, Xiuming
Zhang, Yiming
Hu, Yupiao
Wang, Chengxiao
Xiong, Yin
author_facet Zhang, Zejun
Chen, Lijuan
Cui, Xiuming
Zhang, Yiming
Hu, Yupiao
Wang, Chengxiao
Xiong, Yin
author_sort Zhang, Zejun
collection PubMed
description To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples. The chemical fingerprints of PN were obtained by high performance liquid chromatography. The anti-inflammatory activity was evaluated by the TNF-α and IL-6 inhibiting test. According to the results, raw PN (RPN) displayed a stronger anti-inflammatory effect compared with steamed PN (SPN) samples. Chemometrics analyses showed that notoginsenoside R(1), ginsenosides Rg(1), Re and Rb(1) were the major anti-inflammatory components of RPN, whereas ginsenosides 20(S)-Rg(3) was the active one of SPN treating inflammation. The pharmacologic verification was consistent with the predicted results. In addition, the contents of anti-inflammatory components in RPN were higher than those in SPN, which might be the reason that RPN exhibited stronger effect on the inflammation induced by lipopolysaccharide in RAW264.7 cells. These findings could be beneficial for choosing differential markers for the quality control of RPN and SPN.
format Online
Article
Text
id pubmed-9064609
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90646092022-05-04 Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship Zhang, Zejun Chen, Lijuan Cui, Xiuming Zhang, Yiming Hu, Yupiao Wang, Chengxiao Xiong, Yin RSC Adv Chemistry To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples. The chemical fingerprints of PN were obtained by high performance liquid chromatography. The anti-inflammatory activity was evaluated by the TNF-α and IL-6 inhibiting test. According to the results, raw PN (RPN) displayed a stronger anti-inflammatory effect compared with steamed PN (SPN) samples. Chemometrics analyses showed that notoginsenoside R(1), ginsenosides Rg(1), Re and Rb(1) were the major anti-inflammatory components of RPN, whereas ginsenosides 20(S)-Rg(3) was the active one of SPN treating inflammation. The pharmacologic verification was consistent with the predicted results. In addition, the contents of anti-inflammatory components in RPN were higher than those in SPN, which might be the reason that RPN exhibited stronger effect on the inflammation induced by lipopolysaccharide in RAW264.7 cells. These findings could be beneficial for choosing differential markers for the quality control of RPN and SPN. The Royal Society of Chemistry 2019-06-07 /pmc/articles/PMC9064609/ /pubmed/35520546 http://dx.doi.org/10.1039/c9ra00906j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Zejun
Chen, Lijuan
Cui, Xiuming
Zhang, Yiming
Hu, Yupiao
Wang, Chengxiao
Xiong, Yin
Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title_full Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title_fullStr Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title_full_unstemmed Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title_short Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
title_sort identification of anti-inflammatory components of raw and steamed panax notoginseng root by analyses of spectrum-effect relationship
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064609/
https://www.ncbi.nlm.nih.gov/pubmed/35520546
http://dx.doi.org/10.1039/c9ra00906j
work_keys_str_mv AT zhangzejun identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT chenlijuan identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT cuixiuming identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT zhangyiming identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT huyupiao identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT wangchengxiao identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship
AT xiongyin identificationofantiinflammatorycomponentsofrawandsteamedpanaxnotoginsengrootbyanalysesofspectrumeffectrelationship