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Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship
To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064609/ https://www.ncbi.nlm.nih.gov/pubmed/35520546 http://dx.doi.org/10.1039/c9ra00906j |
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author | Zhang, Zejun Chen, Lijuan Cui, Xiuming Zhang, Yiming Hu, Yupiao Wang, Chengxiao Xiong, Yin |
author_facet | Zhang, Zejun Chen, Lijuan Cui, Xiuming Zhang, Yiming Hu, Yupiao Wang, Chengxiao Xiong, Yin |
author_sort | Zhang, Zejun |
collection | PubMed |
description | To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples. The chemical fingerprints of PN were obtained by high performance liquid chromatography. The anti-inflammatory activity was evaluated by the TNF-α and IL-6 inhibiting test. According to the results, raw PN (RPN) displayed a stronger anti-inflammatory effect compared with steamed PN (SPN) samples. Chemometrics analyses showed that notoginsenoside R(1), ginsenosides Rg(1), Re and Rb(1) were the major anti-inflammatory components of RPN, whereas ginsenosides 20(S)-Rg(3) was the active one of SPN treating inflammation. The pharmacologic verification was consistent with the predicted results. In addition, the contents of anti-inflammatory components in RPN were higher than those in SPN, which might be the reason that RPN exhibited stronger effect on the inflammation induced by lipopolysaccharide in RAW264.7 cells. These findings could be beneficial for choosing differential markers for the quality control of RPN and SPN. |
format | Online Article Text |
id | pubmed-9064609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90646092022-05-04 Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship Zhang, Zejun Chen, Lijuan Cui, Xiuming Zhang, Yiming Hu, Yupiao Wang, Chengxiao Xiong, Yin RSC Adv Chemistry To identify bioactive components of Panax notoginseng (PN) roots in raw and steamed forms, chemometrics analyses including bivariate correlation analysis and partial least squares regression were used to establish the relationships between the chromatograms and anti-inflammatory effect of PN samples. The chemical fingerprints of PN were obtained by high performance liquid chromatography. The anti-inflammatory activity was evaluated by the TNF-α and IL-6 inhibiting test. According to the results, raw PN (RPN) displayed a stronger anti-inflammatory effect compared with steamed PN (SPN) samples. Chemometrics analyses showed that notoginsenoside R(1), ginsenosides Rg(1), Re and Rb(1) were the major anti-inflammatory components of RPN, whereas ginsenosides 20(S)-Rg(3) was the active one of SPN treating inflammation. The pharmacologic verification was consistent with the predicted results. In addition, the contents of anti-inflammatory components in RPN were higher than those in SPN, which might be the reason that RPN exhibited stronger effect on the inflammation induced by lipopolysaccharide in RAW264.7 cells. These findings could be beneficial for choosing differential markers for the quality control of RPN and SPN. The Royal Society of Chemistry 2019-06-07 /pmc/articles/PMC9064609/ /pubmed/35520546 http://dx.doi.org/10.1039/c9ra00906j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhang, Zejun Chen, Lijuan Cui, Xiuming Zhang, Yiming Hu, Yupiao Wang, Chengxiao Xiong, Yin Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title | Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title_full | Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title_fullStr | Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title_full_unstemmed | Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title_short | Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship |
title_sort | identification of anti-inflammatory components of raw and steamed panax notoginseng root by analyses of spectrum-effect relationship |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064609/ https://www.ncbi.nlm.nih.gov/pubmed/35520546 http://dx.doi.org/10.1039/c9ra00906j |
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