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Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels

In this work, manipulating width and equilibrium position of fluorescent microparticles in spiral microchannel fractionation devices by embedding microchambers along the last turn of a spiral is reported. Microchambers with different shapes and sizes were tested at Reynolds numbers between 15.7 and...

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Autores principales: Al-Halhouli, Ala'aldeen, Albagdady, Ahmed, Al-Faqheri, Wisam, Kottmeier, Jonathan, Meinen, Sven, Frey, Lasse Jannis, Krull, Rainer, Dietzel, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064905/
https://www.ncbi.nlm.nih.gov/pubmed/35516901
http://dx.doi.org/10.1039/c9ra03587g
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author Al-Halhouli, Ala'aldeen
Albagdady, Ahmed
Al-Faqheri, Wisam
Kottmeier, Jonathan
Meinen, Sven
Frey, Lasse Jannis
Krull, Rainer
Dietzel, Andreas
author_facet Al-Halhouli, Ala'aldeen
Albagdady, Ahmed
Al-Faqheri, Wisam
Kottmeier, Jonathan
Meinen, Sven
Frey, Lasse Jannis
Krull, Rainer
Dietzel, Andreas
author_sort Al-Halhouli, Ala'aldeen
collection PubMed
description In this work, manipulating width and equilibrium position of fluorescent microparticles in spiral microchannel fractionation devices by embedding microchambers along the last turn of a spiral is reported. Microchambers with different shapes and sizes were tested at Reynolds numbers between 15.7 and 156.6 (100–1000 μL min(−1)) to observe focusing of 2, 5 and 10 μm fluorescent microparticles. This paper also discusses the fabrication process of the microfluidic chips with femtosecond laser ablation on glass wafers, as well as a particle imaging velocimetry (μPIV) study of microparticle trajectories inside a microchamber. It could be demonstrated with an improved final design with inclined microchamber side walls, that the 2 μm particle equilibrium position is shifted towards the inner wall by ∼27 μm and the focusing line's width is reduced by ∼18 μm. Finally, Saccharomyces cerevisiae yeast cells were tested in the final chip and a cell focusing efficiency of 99.1% is achieved.
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spelling pubmed-90649052022-05-04 Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels Al-Halhouli, Ala'aldeen Albagdady, Ahmed Al-Faqheri, Wisam Kottmeier, Jonathan Meinen, Sven Frey, Lasse Jannis Krull, Rainer Dietzel, Andreas RSC Adv Chemistry In this work, manipulating width and equilibrium position of fluorescent microparticles in spiral microchannel fractionation devices by embedding microchambers along the last turn of a spiral is reported. Microchambers with different shapes and sizes were tested at Reynolds numbers between 15.7 and 156.6 (100–1000 μL min(−1)) to observe focusing of 2, 5 and 10 μm fluorescent microparticles. This paper also discusses the fabrication process of the microfluidic chips with femtosecond laser ablation on glass wafers, as well as a particle imaging velocimetry (μPIV) study of microparticle trajectories inside a microchamber. It could be demonstrated with an improved final design with inclined microchamber side walls, that the 2 μm particle equilibrium position is shifted towards the inner wall by ∼27 μm and the focusing line's width is reduced by ∼18 μm. Finally, Saccharomyces cerevisiae yeast cells were tested in the final chip and a cell focusing efficiency of 99.1% is achieved. The Royal Society of Chemistry 2019-06-18 /pmc/articles/PMC9064905/ /pubmed/35516901 http://dx.doi.org/10.1039/c9ra03587g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Al-Halhouli, Ala'aldeen
Albagdady, Ahmed
Al-Faqheri, Wisam
Kottmeier, Jonathan
Meinen, Sven
Frey, Lasse Jannis
Krull, Rainer
Dietzel, Andreas
Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title_full Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title_fullStr Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title_full_unstemmed Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title_short Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
title_sort enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064905/
https://www.ncbi.nlm.nih.gov/pubmed/35516901
http://dx.doi.org/10.1039/c9ra03587g
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