Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis

Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Tanosaki, Sho, Akiyama, Tomohiko, Kanaami, Sayaka, Fujita, Jun, Ko, Minoru S.H., Fukuda, Keiichi, Tohyama, Shugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065422/
https://www.ncbi.nlm.nih.gov/pubmed/35516845
http://dx.doi.org/10.1016/j.xpro.2022.101360
Descripción
Sumario:Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020).

Ejemplares similares