Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis

Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol ca...

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Autores principales: Tanosaki, Sho, Akiyama, Tomohiko, Kanaami, Sayaka, Fujita, Jun, Ko, Minoru S.H., Fukuda, Keiichi, Tohyama, Shugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065422/
https://www.ncbi.nlm.nih.gov/pubmed/35516845
http://dx.doi.org/10.1016/j.xpro.2022.101360
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author Tanosaki, Sho
Akiyama, Tomohiko
Kanaami, Sayaka
Fujita, Jun
Ko, Minoru S.H.
Fukuda, Keiichi
Tohyama, Shugo
author_facet Tanosaki, Sho
Akiyama, Tomohiko
Kanaami, Sayaka
Fujita, Jun
Ko, Minoru S.H.
Fukuda, Keiichi
Tohyama, Shugo
author_sort Tanosaki, Sho
collection PubMed
description Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020).
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spelling pubmed-90654222022-05-04 Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis Tanosaki, Sho Akiyama, Tomohiko Kanaami, Sayaka Fujita, Jun Ko, Minoru S.H. Fukuda, Keiichi Tohyama, Shugo STAR Protoc Protocol Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020). Elsevier 2022-04-28 /pmc/articles/PMC9065422/ /pubmed/35516845 http://dx.doi.org/10.1016/j.xpro.2022.101360 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Tanosaki, Sho
Akiyama, Tomohiko
Kanaami, Sayaka
Fujita, Jun
Ko, Minoru S.H.
Fukuda, Keiichi
Tohyama, Shugo
Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title_full Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title_fullStr Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title_full_unstemmed Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title_short Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
title_sort purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065422/
https://www.ncbi.nlm.nih.gov/pubmed/35516845
http://dx.doi.org/10.1016/j.xpro.2022.101360
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