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Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21–25)(Pal) obtained via electrospinning
Electrospinning technology is useful for making ultrafine drug-eluting fibers for the clinical treatment of wounds. We show the incorporation of an antimicrobial LfcinB-derived peptide into Pullulan nanofibers. The palindromic peptide LfcinB (21–25)(Pal): RWQWRWQWR was synthesized, purified, and cha...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065569/ https://www.ncbi.nlm.nih.gov/pubmed/35514725 http://dx.doi.org/10.1039/c9ra03643a |
Sumario: | Electrospinning technology is useful for making ultrafine drug-eluting fibers for the clinical treatment of wounds. We show the incorporation of an antimicrobial LfcinB-derived peptide into Pullulan nanofibers. The palindromic peptide LfcinB (21–25)(Pal): RWQWRWQWR was synthesized, purified, and characterized by means of the RP-HPLC and MALDI-TOF MS methods. The peptide's antibacterial activity against the E. coli ATCC 25922 strain was evaluated, and the peptide LfcinB (20–25)(Pal) exhibited significant antibacterial activity. Nanofibers were obtained by electrospinning a Pullulan or Pullulan-LfcinB (21–25)(Pal) solution. The obtained nanofibers were characterized via microscopy (AFM and SEM) and RP-HPLC chromatography. The peptide incorporation efficiency was 31%. The Pullulan-LfcinB (21–25)(Pal) nanofibers were soluble in water, and the peptide was liberated immediately. The Pullulan-LfcinB (21–25)(Pal) nanofibers exhibited the same antibacterial activity against E. coli strain as the free peptide LfcinB (21–25)(Pal). The results suggest that Pullulan-LfcinB (21–25)(Pal) nanofibers could be considered for designing and developing antibacterial wound dressings. |
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