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Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization

Biosurfactants have been well recognized as an environmentally friendly alternative to chemical surfactants. However, their production remains challenging due to low productivity, short-term microbe stability and the potentially toxic by-products generated in the growth media. To overcome these chal...

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Autores principales: Zhu, Zhiwen, Zhang, Baiyu, Chen, Bing, Ling, Jingjing, Cai, Qinghong, Husain, Tahir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065571/
https://www.ncbi.nlm.nih.gov/pubmed/35514694
http://dx.doi.org/10.1039/c9ra02784j
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author Zhu, Zhiwen
Zhang, Baiyu
Chen, Bing
Ling, Jingjing
Cai, Qinghong
Husain, Tahir
author_facet Zhu, Zhiwen
Zhang, Baiyu
Chen, Bing
Ling, Jingjing
Cai, Qinghong
Husain, Tahir
author_sort Zhu, Zhiwen
collection PubMed
description Biosurfactants have been well recognized as an environmentally friendly alternative to chemical surfactants. However, their production remains challenging due to low productivity, short-term microbe stability and the potentially toxic by-products generated in the growth media. To overcome these challenges, the emerging biofilm-based biosynthesis was investigated in this study. A fresh insight into the biosynthesis process was provided through using waste fly ash as a carrier material. The biofilm produced by biosurfactant producer B. subtilis N3-1P attached onto the surface of fly ash acted as a robust and effective biocatalyst. Zeta potential analysis and scanning electron microscope (SEM) characterization were conducted to help unravel the biocatalyst formation. High-value biosurfactant products were then produced in an efficient and sustainable manner. Stimulation by a fly ash assisted biocatalyst on biosurfactant production was confirmed. The biosurfactant yield was boosted over ten times after 24 hours, at a fly ash dosage of 0.5%. The highest biosurfactant yield was achieved after five days, with a final productivity of 305 critical micelle dilution. The underlying mechanism of fly ash assisted biosurfactant production was tracked through it exerting an effect on the quorum sensing system. Fourier-transform infrared (FTIR) spectroscopy and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis demonstrated that the final biosurfactant product belonged to the lipopeptides. This research output is expected to accelerate the development of more effective bioreactors, and make a continuous contribution to high-value product generation and waste reduction.
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spelling pubmed-90655712022-05-04 Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization Zhu, Zhiwen Zhang, Baiyu Chen, Bing Ling, Jingjing Cai, Qinghong Husain, Tahir RSC Adv Chemistry Biosurfactants have been well recognized as an environmentally friendly alternative to chemical surfactants. However, their production remains challenging due to low productivity, short-term microbe stability and the potentially toxic by-products generated in the growth media. To overcome these challenges, the emerging biofilm-based biosynthesis was investigated in this study. A fresh insight into the biosynthesis process was provided through using waste fly ash as a carrier material. The biofilm produced by biosurfactant producer B. subtilis N3-1P attached onto the surface of fly ash acted as a robust and effective biocatalyst. Zeta potential analysis and scanning electron microscope (SEM) characterization were conducted to help unravel the biocatalyst formation. High-value biosurfactant products were then produced in an efficient and sustainable manner. Stimulation by a fly ash assisted biocatalyst on biosurfactant production was confirmed. The biosurfactant yield was boosted over ten times after 24 hours, at a fly ash dosage of 0.5%. The highest biosurfactant yield was achieved after five days, with a final productivity of 305 critical micelle dilution. The underlying mechanism of fly ash assisted biosurfactant production was tracked through it exerting an effect on the quorum sensing system. Fourier-transform infrared (FTIR) spectroscopy and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis demonstrated that the final biosurfactant product belonged to the lipopeptides. This research output is expected to accelerate the development of more effective bioreactors, and make a continuous contribution to high-value product generation and waste reduction. The Royal Society of Chemistry 2019-06-28 /pmc/articles/PMC9065571/ /pubmed/35514694 http://dx.doi.org/10.1039/c9ra02784j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhu, Zhiwen
Zhang, Baiyu
Chen, Bing
Ling, Jingjing
Cai, Qinghong
Husain, Tahir
Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title_full Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title_fullStr Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title_full_unstemmed Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title_short Fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
title_sort fly ash based robust biocatalyst generation: a sustainable strategy towards enhanced green biosurfactant production and waste utilization
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065571/
https://www.ncbi.nlm.nih.gov/pubmed/35514694
http://dx.doi.org/10.1039/c9ra02784j
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